主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (3): 254-259.doi: 10.3969/j.issn.1006-2157.2019.03.012

• 针灸 • 上一篇    下一篇

眼针与体针对脑缺血再灌注损伤大鼠24 h脑组织水通道蛋白4表达的差异研究

王哲1, 樊立林2, 潘茜1, 马贤德1, 张威1, 赵金茹1, 高原1, 关洪全1#   

  1. 1 辽宁中医药大学基础医学院 辽宁 110847;
    2 中国医科大学盛京医院神经外科
  • 收稿日期:2018-09-22 出版日期:2019-03-30 发布日期:2019-04-02
  • 通讯作者: # 关洪全,男,教授,博士生导师,主要研究方向:中医药对免疫的调控,E-mail:hongquanguan@sina.com
  • 作者简介:王哲,女,博士,教授,硕士生导师
  • 基金资助:
    国家重点基础研究发展计划(973计划)资助项目(No.2007CB512702),国家自然科学基金面上资助项目(No.81673925),辽宁省自然科学基金资助项目(No.201602507)

Differences in effects of eye acupuncture and body acupuncture on 24 h expression of cerebral aquaporin 4 in rats with cerebral ischemia-reperfusion injury*

Wang Zhe1, Fan Lilin2, Pan Qian1, Ma Xiande1, Zhang Wei1, Zhao Jinru1, Gao Yuan1, Guan Hongquan1#   

  1. 1 School of Basic Medicine, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Department of Neurosurgery, Shengjing Hospital of China Medical University, Liaoning 110004, China
  • Received:2018-09-22 Online:2019-03-30 Published:2019-04-02
  • Contact: Prof. Guan Hongquan, Doctoral supervisor. School of Basic Medicine, Liaoning University of Traditional Chinese Medicine. E-mail: hongquanguan@sina.com
  • Supported by:
    Subject of National Key Basic Research Development Plan (No. 2007CB512702), National Natural Science Foundation of China (No. 81673925), National Natural Science Foundation of Liaoning Province (No. 201602507)

摘要: 目的 观察眼针与体针对24 h脑缺血再灌注损伤模型(CIRI)大鼠脑组织水通道蛋白4(AQP4)表达的影响,探讨眼针与体针治疗急性脑缺血再灌注损伤机制的差异。方法 SD大鼠48只,采用随机数字法分为6组,分别为:正常对照组,假手术组,模型组,穴区组,体针组,穴区外组。模型组、穴区组、体针组、穴区外组大鼠采用线栓法制备脑缺血再灌注损伤模型。缺血1 h,拔出栓塞线,令其再灌注。再灌注后1 h,大鼠完全苏醒后,采用ZeaLonga评分法进行大鼠神经功能评分,并开始给予针刺干预,再灌注24 h,处死大鼠,采用电镜观察脑组织神经细胞形态学变化,采用Western blot方法和实时荧光定量聚合酶链反应(RQ-PCR)方法检测脑组织中水通道蛋白4(AQP4)以及mRNA表达水平。结果 与模型组比较,穴区组大鼠神经功能缺损评分明显下降;穴区组大脑皮质神经元细胞电镜下体积增大,核大,胞质丰富,线粒体、粗面内质网等肿胀明显减轻。脑组织AQP4蛋白及mRNA表达明显下调(P<0.05)。眼针与体针比较差异无统计学意义。结论 眼针与体针均具有改善大鼠24 h脑缺血再灌注损伤的作用;其机制与脑组织AQP4表达下调有关,眼针与体针差异不明显。

关键词: 眼针疗法, 体针疗法, 脑缺血再灌注损伤, 水通道蛋白4, 大鼠

Abstract: Objective To observe the influence of eye acupuncture and body acupuncture on 24 h expression of cerebral aquaporin 4 (AQP4) in rats with cerebral ischemia-reperfusion injury (CIRI), and discuss the difference in mechanism of treating CIRI between eye acupuncture and body acupuncture. Methods SD rats (n=48) were divided into 6 groups by using random digital method: normal control group, 24 h sham-operation group, 24 h model group, 24 h eye acupuncture group, 24 h body acupuncture group and 24 h outside area group. The model of CIRI was established by using thread approach in 24 h model group, 24 h eye acupuncture group, 24 h body acupuncture group and 24 h outside area group. After making ischemia for 1 s, the thread was pulled out to make reperfusion. After 1 h and rats revived fully, the neurologic function was scored by applying ZeaLonga scoring method, and acupuncture intervention was given to rats. Then reperfusion was continued for 24 h and rats were executed. The changes of cerebral neurocyte morphology were observed by using microscope, and protein and mRNA expressions of AQP4 were detected by using Western blotting assay and RQ-PCR. Results The neurological functional deficit scores decreases significantly in eye acupuncture group compared with model group. In eye acupuncture group cerebral cortex neurons increased in volume with large cores and abundant cytoplasm, and swelling of mitochondria and rough endoplasmic reticulum were significantly relieved. The protein and mRNA expressions of AQP4 decreased significantly (P<0.05). The comparison showed that there was no difference between eye acupuncture group and the body acupuncture group. Conclusion Eye acupuncture and body acupuncture have relieving effects on 24 h CIRI, and the mechanism is related to decrease of brain AQP4 expression. The difference between eye acupuncture and body acupuncture is not significant.

Key words: eye acupuncture therapy, body acupuncture therapy, cerebral ischemia-reperfusion injury, aquaporin 4, rats

中图分类号: 

  • R245.3