主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (5): 404-408.doi: 10.3969/j.issn.1006-2157.2019.05.008

• 中药药理 • 上一篇    下一篇

补中益气颗粒对EAT大鼠Treg/Th17细胞因子表达的影响*

刘守尧1, 关青青2, 韩静1,3, 袁泉1, 曹丽双4, 刘昕怡1,3, 夏仲元1#   

  1. 1 中日友好医院 北京 100029;
    2 开封市人民医院;
    3 北京中医药大学;
    4 北京市朝阳区望京社区卫生服务中心
  • 收稿日期:2018-11-20 出版日期:2019-05-30 发布日期:2019-06-05
  • 通讯作者: 夏仲元, 女, 博士, 主任医师, 博士生导师, 研究方向:中西医结合诊治甲状腺、乳腺疾病, E-mail:2718421708@qq.com
  • 作者简介:刘守尧,男,博士,主治医师
  • 基金资助:
    *首都特色临床应用研究资助项目(No.Z181100001718141),北京市中医药科技项目(No.JJ2014-66)

Influence of BuzhongYiqi Granule on expressions of Treg/Th17 cytokines in rats with experimental autoimmune thyroiditis*

Liu Shouyao1, Guan Qingqing2, Han Jing1,3, Yuan Quan1, Cao Lishuang4, Liu Xinyi1,3, Xia Zhongyuan1#   

  1. 1 China-Japan Friendship Hospital, Beijing 100029, China;
    2 People’s Hospital of Kaifeng City, Henan 475001, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China;
    4 Wangjing Community Health Service Center, Chaoyang District, Beijing 100102, China
  • Received:2018-11-20 Online:2019-05-30 Published:2019-06-05
  • Contact: Xia Zhongyuan, Ph. D., Chief Physician, Doctoral Supervisor. China-Japan Friendship Hospital, No. 2, Yinghua Donglu Road, Chaoyang District, Beijing 100029. E-mail: 2718421708@qq.com
    Funding: Capital Characteristic Clinical Application Research (No. Z181100001718141), Beijing Project of Traditional Chinese Medical Science and Technology (No. JJ2014-66)

摘要: 目的 探讨补中益气颗粒对实验性自身免疫性甲状腺炎(EAT)大鼠调节性T细胞(Treg)/辅助性T细胞17(Th17)细胞因子表达的影响,从Treg/Th17细胞因子失衡角度探讨其治疗桥本氏甲状腺炎的机制。方法 运用猪甲状腺球蛋白与完全弗氏佐剂和不完全弗氏佐剂混合免疫注射法及高碘喂养法复制EAT大鼠模型,随机分为空白组、模型组、补中益气组、优甲乐组、补中益气+优甲乐组,药物干预2个月后,取大鼠脾脏组织,采用荧光定量PCR、蛋白质印迹(Western blot)法分别检测大鼠脾脏转化生长因子-β(TGF-β)、叉头翼状螺旋转录因子(Foxp3)、维甲酸相关孤儿受体γT(RORγT)、白细胞介素-17(IL-17)基因和蛋白表达水平。结果 与空白组比较,模型组RORγT、IL-17基因和蛋白表达上调(P<0.05),Foxp3、TGF-β基因表达减少(P<0.05),蛋白表达无统计学意义(P>0.05)。与模型组比较,补中益气组、优甲乐组、补中益气+优甲乐组RORγT、IL-17基因表达下调(P<0.05),蛋白表达减少,RORγT改变具有统计学意义(P<0.05);Foxp3、TGF-β基因表达上调(P<0.05),蛋白表达无统计学意义(P>0.05)。药物各组组间比较,仅优甲乐组Foxp3蛋白表达高于补中益气组(P<0.05),RORγT、IL-17、TGF-β蛋白和基因表达3组间无统计学意义。结论 补中益气颗粒能够抑制甲状腺自身免疫性炎症,其作用机制可能与调节Treg/Th17相关细胞因子的平衡状态有关。

关键词: 补中益气颗粒, 桥本氏甲状腺炎, 炎症因子, 大鼠

Abstract: Objective To investigate the influence of BuzhongYiqi(Middle-tonifying Qi-replenishing) Granule (BZYQ Granule) on expressions of Treg/Th17 cytokines in rats with experimental autoimmune thyroiditis (EAT), and discuss the mechanism of BZYQ Granule in treatment of Hashimoto’s thyroiditis from the aspect of unbalance of Treg/Th17 cytokines. Methods EAT rat model was established by using mixed immune injection of porcine thyroglobulin and complete and incomplete Freund’s adjuvant and high iodine feeding, and then the rats were randomly divided into blank group, model group, BZYQ Granule group, Euthyrox group and BZYQ Granule+Euthyrox group. After medicinal intervention for 2 months, the samples of rat spleen tissue were collected for detecting the protein and mRNA expressions of transforming growth factor-β (TGF-β), Foxp3 transcription factor (Foxp3), retinoic acid-related orphan receptor γT (RORγT) and interleukin-17 (IL-17) by using RT-qPCR and Western blotting assay. Results Compared with blank group, the protein and mRNA expressions of RORγT and IL-17 were up-regulated (P<0.05), and mRNA expressions of Foxp3 and TGF-β were down-regulated (P<0.05) but protein expressions of them had no statistical significance (P>0.05) in model group. Compared with model group, the mRNA expressions of RORγT and IL-17 were down-regulated (P<0.05) and protein expressions of them decreased, and the expression of RORγT had statistical significance(P<0.05) but expression of IL-17 had no statistical significance in all medicinal groups. The mRNA expressions of Foxp3 and TGF-β were up-regulated (P<0.05) and protein expressions of them had statistical significance (P>0.05) in all medicinal groups compared with model group. The comparison among medicinal groups showed that the mRNA expressions of RORγT, IL-17, Foxp3 and TGF-β had no significant difference among these groups, while protein expression of Foxp3 was higher in Euthyrox group than that in BZYQ Granule group (P<0.05). The protein expressions of RORγT, IL-17 and TGF-β had no significant difference among BZYQ Granule group, Euthyrox group and BZYQ Granule+Euthyrox group. Conclusion BZYQ Granule can inhibit thyroid autoimmune inflammatory, and the mechanism may be related to the regulation of balance status of Treg/Th17 cytokines.

Key words: BuzhongYiqi(Middle-tonifying Qi-replenishing) Granule, Hashimoto’s thyroiditis, inflammatory factors, rats

中图分类号: 

  • R285.5