主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (5): 416-422.doi: 10.3969/j.issn.1006-2157.2019.05.010

• 科技之窗 • 上一篇    下一篇

蒙药协日嘎-4对肾损伤模型大鼠的保护作用*

李斌鑫1,2, 嵇增云1,2, 董玉1,2#, 白凤1,2, 朱小玲2,3, 孙国园2,3, 王玥璇4   

  1. 1 内蒙古医科大学药学院 内蒙古 010110;
    2 内蒙古自治区蒙药药效物质与质量控制工程技术研究中心;
    3 内蒙古自治区国际蒙医医院;
    4 呼和浩特市第二中学
  • 收稿日期:2019-01-07 出版日期:2019-05-30 发布日期:2019-06-05
  • 通讯作者: 董玉, 女, 博士, 教授, 博士生导师, 研究方向:中蒙药(复方)有效物质基础研究及药物创新, E-mail: dongyu010@126.com
  • 作者简介:李斌鑫,男,在读硕士生
  • 基金资助:
    *国家自然科学基金资助项目(No.81660703),内蒙古自治区科技计划重点项目(No.1615001)

Protective effect of Mongolian medicinal Xieri GA-4 in rat model of kidney injury*

Li Binxin1,2, Ji Zengyun1,2, Dong Yu1,2#, Bai Feng1,2, Zhu Xiaoling2,3, Sun Guoyuan2,3, Wang Yuexuan4   

  1. 1 College of Pharmacy, Inner Mongolian Medical University, Inner Mongolia 010110, China;
    2 Inner Mongolia Engineering Research Center for Effective Substances and Quality Control of Traditional Mongolian Medicine, Inner Mongolia 010110, China;
    3 International Hospital of Mongolian Medicine, Inner Mongolia 010065, China;
    4 Second Middle School of Hohhot City, Inner Mongolia 010070, China
  • Received:2019-01-07 Online:2019-05-30 Published:2019-06-05
  • Contact: Prof. Dong Yu, Ph. D., Doctoral supervisor. College of Pharmacy, Inner Mongolia Medical University, Jinshan Development Zone, Hohhot 010110. E-mail: dongyu010@126.com

摘要: 目的 研究协日嘎-4对庆大霉素致急性肾损伤模型大鼠的保护作用。方法 将健康雄性Wistar大鼠随机分为空白组、模型组、协日嘎-4低剂量组、协日嘎-4中剂量组、协日嘎-4高剂量组、肾炎消肿片组、强的松组,除空白组外,所有大鼠皮下注射庆大霉素[80 mg/(kg·d)]诱导急性肾损伤,连续6 d。造模结束2 h后分别给予相应药物治疗,连续给药14 d,给药结束后,用酶联免疫吸附法(ELISA)检测血清肌酐(Scr)、血清尿素氮(BUN)、尿蛋白(UP)、尿酶(NAG)、肾损伤因子(KIM-1)的表达水平。计算各组肾脏指数(KI)和前列腺指数(PI),用苏木素-伊红(HE)染色处理肾组织切片,光镜观察组织病理学改变。结果 与空白组相比,模型组大鼠尿量增加(P<0.01),Scr、BUN、UP、NAG、KIM-1的含量均升高(P<0.01),KI、PI也有不同程度的升高(P<0.01)。与模型组相比,协日嘎-4低剂量组可较快的降低 Scr、BUN、UP、PI 的表达水平(P<0.01),持续用药至一定血药浓度可降低 NAG、KIM-1 的表达水平(P<0.01),协日嘎-4中、高剂量组可降低庆大霉素致肾损伤模型大鼠的尿量(P<0.05、P<0.01),降低Scr、BUN、UP、NAG、KIM-1、KI、PI的表达水平(P<0.05、P<0.01)。阳性对照药在疾病发生的一定时间段内可降低某些因子的表达水平,但在整个实验过程中,协日嘎-4治疗组要优于阳性对照组。协日嘎-4低、中、高剂量组肾小球系膜细胞增生改善、肾小管上皮细胞脱落现象明显得到抑制。结论 协日嘎-4对庆大霉素致肾损伤模型大鼠有保护作用,并对肾损伤导致的 PI 增大有一定的治疗效果。

关键词: 协日嘎-4, 庆大霉素, 肾损伤大鼠模型, 前列腺指数, 保护作用

Abstract: Objective To study the protective effect of Mongolian medicinal Xieri GA-4 in rat model of acute kidney injury induced by gentamicin. Methods Male healthy Wistar rats were randomly divided into blank group, model group, low-dose, mid-dose, high-dose Xieri GA-4 groups, group of Shenyan Xiaozhong Pian (Nephritis Swelling-relieving Tablet, SYXZ Tablet group) and prednison group. Except of blank group, other groups were given hypodermic injection of gentamicin (80 mg/kg·d) for reducing acute kidney injury for 6 d, and were treated with corresponding medicianls after 2 h for 14 d. The levels of serum creatinine (SCr), blood urea nitrogen (BUN), urine protein (UP), uracil-N-glycosylase (NAG) and kidney injury molecular (KIM-1) were detected by using ELISA after treatment. The kidney index (KI) and prostatic index (PI) were calculated in all groups. The histopathological changes of kidney tissue were observed with light microscope after HE staining. Results Compared with blank group, the urine volume(P<0.01), and levels of Scr, BUN, UP, NAG and KIM-1 (P<0.01) increased, and expressions of KI and PI (P<0.01) also increased in model group. Compared with model group, the levels of Scr, BUN and UP and PI decreased quickly(P<0.01), and expressions of NAG and KIM-1 were reduced after medicinal reaching to certain dose after continuous administration in low-dose Xieri GA-4 group(P<0.01). The urine volume, and levels of Scr, BUN, UP, NAG and KIM-1(P<0.05, P<0.01), and expressions of KI and PI decreased in mid-dose and high-dose Xieri GA-4 groups(P<0.05, P<0.01). The levels of some factors decreased in prednison group and SYXZ Tablet group in a period, but the results were better in all Xieri GA-4 groups during the whole experimental course. The proliferation of mesangial cells was relieved and tubular desquamation was significantly inhibited in low-dose, mid-dose, high-dose Xieri GA-4 groups. Conclusion Xieri GA-4 has a protective effect on rat model of kidney injury induced by gentamicin, and has therapeutic effect on PI increase induced by kidney injury.

Key words: Xieri GA-4, gentamicin, rat model of kidney injury, prostatic index, protective effect

中图分类号: 

  • R285.5