主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (7): 549-554.doi: 10.3969/j.issn.1006-2157.2019.07.005

• 科技之窗 • 上一篇    下一篇

肝郁证雌雄大鼠神经-内分泌变化的比较*

钱梦, 谢鸣#, 张媛凤, 李聪, 刘碧原, 赵荣华   

  1. 北京中医药大学中医学院 北京 100029
  • 收稿日期:2019-01-25 出版日期:2019-07-30 发布日期:2019-09-03
  • 通讯作者: 谢鸣,男,博士,教授,博士生导师,主要研究方向:方-证相关研究,E-mail:xieming603@263.net
  • 作者简介:钱梦,女,在读博士生
  • 基金资助:
    国家自然科学基金资助项目(No. 81173193)

Comparison of neuroendocrine changes in male and female rat models of liver stagnation pattern*

Qian Meng, Xie Ming#, Zhang Yuanfeng, Li Cong, Liu Biyuan, Zhao Ronghua   

  1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2019-01-25 Online:2019-07-30 Published:2019-09-03
  • Contact: Prof. Xie Ming, male, Ph.D., Doctoral supervisor, Beijing University of Chinese Medicine, Beijing 10029. E-mail: xieming603@263.net

摘要: 目的 比较肝郁证模型雌、雄大鼠的神经-内分泌变化以探讨肝郁证现代内涵的性别差异。方法 大鼠适应性喂养1 周后,雄鼠与动情周期稳定的雌鼠分别随机分为正常组和模型组(每组各10只),模型组采用慢性束缚应激法复制肝郁证模型,连续28 d。实验29~30 d处杀取材,测定各组大鼠神经-内分泌相关指标:单胺类递质,血浆5-羟色胺(5-HT)、肾上腺素(E)、去甲肾上腺素(NE);肾上腺皮质轴(HPA),下丘脑促肾上腺皮质激素释放激素(CRH)、血清促肾上腺皮质激素(ACTH)、皮质酮/肾上腺酮(CORT);性腺轴(HPG),下丘脑促性腺激素释放激素(GnRH)、血清卵泡刺激素(FSH)、促黄体生成素(LH)、泌乳素(PRL)、睾酮(T)、雌二醇(E2);甲状腺轴(HPT),下丘脑促甲状腺激素释放激素(TRH)、血清促甲状腺激素(TSH)、三碘甲腺原氨酸(T3)、甲状腺素(T4)。结果 与正常组雄鼠比较,模型组雄鼠血浆5-HT、NE及E明显升高(P<0.01);下丘脑CRH与血清CORT显著升高(P<0.05),血清ACTH无明显变化(P>0.05);血清FSH、LH、T显著降低(P<0.05,P<0.01),PRL、E2显著升高(P<0.05,P<0.01);TSH显著升高(P<0.05),T3、T4显著降低(P<0.05),下丘脑TRH无明显变化(P>0.05)。 与正常组雌鼠比较,模型组雌鼠血浆E显著升高(P<0.05),5-HT、NE显著降低(P<0.05,P<0.01);下丘脑CRH与血清ACTH、CORT显著升高(P<0.01);下丘脑GnRH和血清FSH、LH、PRL、T及E2均显著升高(P<0.01);下丘脑TRH与血清T3、T4显著升高(P<0.01),TSH显著降低(P<0.01)。表明肝郁模型雌、雄大鼠均存在HPA轴的亢进,雌性还存在外周单胺类递质的代谢紊乱,HPT轴和HPG轴功能的亢进;雄性存在交感神经功能亢进,HPT轴和HPG轴功能的抑制。结论 慢性束缚法肝郁证模型雌、雄大鼠均存在神经-内分泌功能的异常,但两者各有特点,推测中医肝郁证的现代病生理学内涵因性别而有所差异。

关键词: 肝郁证, 雌雄大鼠, 肾上腺轴, 性腺轴, 甲状腺轴

Abstract: Objective To explore the gender differences of the modern connotation of liver stagnation pattern by comparing changes in neuro-endocrine of female and male rat models of liver stagnation pattern. Methods One week after adaptive feeding in rats, male and female rats with stable estrous cycle were randomly divided into normal group and model group (n=10 in each group). Chronic restraint stress method for 28 consecutive days was used to replicate liver stagnation pattern model At 29th-30th days of the experiment, all rats were sacrificed and neuro-endocrine related parameters of each group were measured: Monoamine transmitter index: plasma 5-hydroxytryptamine (5-HT), adrenaline (E) and norepinephrine (NE); Adrenal axis index: hypothalamic corticotropin-releasing hormone (CRH), serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT); Gonadal axis index: hypothalamic gonadotropin releasing hormone (GnRH) , Serum Follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), testosterone (T), estradiol (E2); Thyroid axis index: hypothalamic thyrotropin-releasing hormone (TRH), Serum thyroid-stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4). Results Compared with male rats in the normal group, plasma 5-HT, NE and E of male rats in the model group were significantly increased (P<0.01); hypothalamus CRH and serum CORT were significantly increased (P<0.05), while serum ACTH didn’t change significantly (P>0.05); serum FSH, LH and T were significantly decreased (P<0.05, P<0.01), whereas serum PRL and E2 were significantly increased (P<0.05, P<0.01); serum TSH was significantly increased (P<0.05); serum T3 and T4 were significantly decreased (P<0.05), and hypothalamic TRH didn’t change significantly (P>0.05). Compared with female rats in the normal group, plasma E of female rats in the model group was significantly increased (P<0.05); 5-HT and NE were significantly decreased (P<0.05, P<0.01); hypothalamus CRH and serum ACTH, CORT were significantly increased (P<0.01); hypothalamus GnRH, serum FSH, LH, PRL, T and E2 were significantly increased (P<0.01); TRH of the hypothalamus, serum T3 and T4 were significantly increased (P<0.01), and serum TSH was significantly decreased (P<0.01). The results showed that both the female and male rats of the liver stagnation model had hyperactivity of the HPA axis. Female rats also had metabolic disorders of peripheral monoamine neurotransmitters and hyperfunction of HPT and HPG axis. Male rats had sympathetic hyperfunction, inhibition of the function of HPT and HPG axis. Conclusion Female and male rats with liver stagnation models have different neuro-endocrine function abnormalities. It is speculated that the modern physiological connotation of liver stagnation pattern varies according to gender.

Key words: liver stagnation syndrome, male and female rats, HPA, HPG, HPT

中图分类号: 

  • R241.6