主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (7): 577-583.doi: 10.3969/j.issn.1006-2157.2019.07.009

• 中药药理 • 上一篇    下一篇

消溃方灌肠治疗溃疡性结肠炎模型大鼠的作用机制研究*

张慧俭1, 周鹏飞1, 刘世举2#, 刘佃温2, 张新春2   

  1. 1 河南中医药大学 河南 450000;
    2 河南中医药大学第三附属医院
  • 收稿日期:2019-01-25 出版日期:2019-07-30 发布日期:2019-09-03
  • 通讯作者: 刘世举,男,硕士,副主任医师,主要研究方向:中医药防治肛肠疾病的研究,E-mail:liusj120@126.com
  • 作者简介:张慧俭,女,硕士,讲师
  • 基金资助:
    国家自然科学基金资助项目(No. 81473435), 河南省科技攻关项目(No.162102310109),河南省中医药科学研究项目(No.2018ZY2055)

Mechanism of Xiaokui enema formula in the treatment of rat ulcerative colitis*

Zhang Huijian1, Zhou Pengfei1, Liu Shiju2#, Liu Dianwen2 , Zhang Xinchun2   

  1. 1 Henan University of Chinese Medicine, Henan 450000, China;
    2 No.3 Affiliated Hospital of Henan University of Traditional Chinese Medicine, Henan 450003, China
  • Received:2019-01-25 Online:2019-07-30 Published:2019-09-03
  • Contact: A/Rrof. Liu Shiju, master, Graduate supervisor, Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, No. 63 Dongming Road, Zhengzhou 450003, E-mail: Liusj120@126.com.

摘要: 目的 研究消溃方对溃疡性结肠炎(UC)模型大鼠TH17/Treg平衡靶点的调节作用,对FOXP3、RORγt的表达和细胞因子白细胞介素-17(IL-17)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)和TGF-β表达的影响。方法 通过三硝基苯磺酸-乙醇灌肠法建立溃疡性结肠炎大鼠模型,分别采用美沙拉秦灌肠液(0.7 g/kg)和消溃方高、低剂量(18.3、9.15 g/kg)进行灌肠治疗;治疗完成后,采用流式细胞术检测大鼠外周血Treg和Thl7细胞占总CD4+T细胞百分比,酶联免疫吸附法(ELISA)分析大鼠血清中IL-17、IL-6、IL-10和TGF-β水平,Western blot方法检测结肠组织中Treg转录因子FOXP3和Th17转录因子RORγt的表达水平。结果 美沙拉秦组和消溃方高、低剂量组治疗后均能够降低促炎因子IL-17、IL-6的浓度,提高IL-10和TGF-β的浓度,且消溃方高、低剂量组和美沙拉秦组均能提高FOXP3的表达,降低RORγt的表达,达到抗炎、促进UC溃疡面愈合的作用,并且消溃方高剂量组效果优于消溃方低剂量组和美沙拉秦组。结论 消溃方能促进溃疡性结肠炎大鼠结肠组织中Treg细胞分化生成,介导免疫耐受程度,抑制Th17细胞分化,调节Th17/Treg平衡,维持体内免疫平衡,减轻肠道炎症反应,从而起到治疗溃疡性结肠炎的作用。

关键词: 溃疡性结肠炎, Th17/Treg, 消溃方, 灌肠治疗, 大鼠

Abstract: Objective To investigate the effects of Xiaokuifang (Ulcer-curing Formula, XKF) on TH17/Treg signal pathways and the expression of FOXP3、RORγt and cytokine IL-17, IL-6, IL-10, and TGF-β in rats with ulcerative colitis. Methods Rat model of ulcerative colitis was established by using enema administration with TNBS and ethanol. The mesalazine(0.7 g/kg), high-dose (18.3 g/kg) and low-dose (9.15 g/kg) XKF were administered in enema to model rats divided into different groups. After enema, rat peripheral blood Thl7 and Treg cells of CD4+ Tcell percentage were measured with flow cytometry; IL-17、IL-6、IL-10 and TGF-β level in the rat serum were tested by using enzyme-linked immunosorbent (ELISA), colon tissue of Treg transcription factor FOXP3 and Th17 transcription factor RORγt gamma expression level were detected with Western blot assay. Results Both the mesalazine and high and low-dose of XKF groups could reduce the concentration of proinflammatory factor IL-17 and IL-6, and improve the concentration of IL-10 and TGF-β after treatment, high and low-dose XKF group and mesalazine group could improve the expression of FOXP3, reduce RORγt expression, and contribute to the effect of anti-inflammatory promote healing of ulcer surface. And the high-dose group was better than both the low-dose group and mesalazine group at the same time. Conclusion Xiaokuifang could promote Treg cells in rat colon tissue differentiation, mediate immune tolerance levels, inhibit the differentiation of Th17 cells, regulate the balance of Th17/Treg, maintain an immune balance, reduce the inflammatory response and play a role in the treatment of ulcerative colitis.

Key words: ulcerative colitis, Th17/Treg, Xiaokuifang, enema therapy, rats

中图分类号: 

  • R285.5