主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2019, Vol. 42 ›› Issue (11): 910-915.doi: 10.3969/j.issn.1006-2157.2019.11.006

• 中药药理 • 上一篇    下一篇

二补助育汤对胚胎着床障碍模型小鼠子宫内膜自噬基因Beclin-1、LC3B表达的影响*

梁嘉玲1,2, 刘雁峰1#, 申萌萌3, 潘雪1,2, 徐秀丽1,2, 谢宝珍1,2, 郑涵予2   

  1. 1 北京中医药大学东直门医院 北京 100700;
    2 北京中医药大学;
    3 北京中医药大学第三附属医院
  • 收稿日期:2019-05-13 出版日期:2019-11-30 发布日期:2019-12-03
  • 通讯作者: 刘雁峰,女,博士,教授,博士生导师,研究方向:不孕症与更年期综合征的中医治疗,E-mail:liuyaf8888@sina.com
  • 作者简介:梁嘉玲,女,在读博士生
  • 基金资助:
    * 国家自然科学基金面上项目(No.81473721),北京中医药大学在读研究生自主课题(No.2018-JYBZZ-XS139)

Influence of Erbu Zhuyu Tang on expressions of endometrial autophagy genes-Beclin-1 and LC3B in mice with embryo implantation dysfunction*

Liang Jialing1,2, Liu Yanfeng1#, Shen Mengmeng3, Pan Xue1,2, Xu Xiuli1,2, Xie Baozhen1,2, Zheng Hanyu2   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Beijing University of Chinese Medicine, Beijing 100029, China;
    3 Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2019-05-13 Online:2019-11-30 Published:2019-12-03
  • Contact: Prof. Liu Yanfeng, Ph.D., Doctoral supervisor. Department of Gynecology, Dongzhimen Hospital, Beijing University of Chinese Medicine, No. 5, Haiyuncang Street, Dongcheng District, Beijing 100700. E-mail: liuyaf8888@sina.com

摘要: 目的 探讨二补助育汤对胚胎着床障碍模型小鼠胚胎着床率及子宫内膜自噬基因Beclin-1、微管相关蛋白轻链3(LC3B)mRNA及蛋白表达的影响。方法 将50只妊娠ICR雌性小鼠随机分为空白组、模型组、二补助育汤低剂量组、二补助育汤高剂量组、阿司匹林组,每组10只。用米非司酮造成胚胎着床障碍模型,每组每日以相应药物灌胃,于妊娠第5天下午脱颈处死,观察各组子宫形态,计算各组妊娠率、平均着床位点数,检测子宫内膜Beclin-1、LC3B mRNA及蛋白的表达量。结果 空白组小鼠子宫色泽红润,胚胎着床部位膨大,分布均匀;模型组小鼠子宫色白、较细;用药各组子宫较细,色泽淡红。模型组平均着床位点数较空白组减少(P<0.001);与模型组相比,二补助育汤高剂量组与阿司匹林组平均胚胎着床位点数均增多(P<0.05)。模型组小鼠子宫内膜Beclin-1、LC3B mRNA及蛋白表达水平均低于空白组(P<0.05);与模型组相比,二补助育汤低、高剂量组与阿司匹林组Beclin-1、LC3B mRNA及蛋白表达水平均增加(P<0.05)。结论 二补助育汤可增加Beclin-1、LC3B mRNA及蛋白的表达,提高子宫内膜细胞自噬水平,从而改善子宫内膜容受性增加平均着床位点数。

关键词: 二补助育汤, 胚胎着床障碍, 子宫内膜容受性, 自噬, 小鼠

Abstract: Objective To investigate the influence of Erbu Zhuyu Tang (Decoction of Promoting Fertility with Two Tonifying Medicinals) on embryo implantation rate and mRNA and protein expressions of endometrial autophagy genes-Beclin-1 and microtubule-associated protein light chain 3 (LC3B) in mice with embryo implantation dysfunction. Methods Female ICR mice (n=50) were randomly divided into blank group, model group, low-dose and high-dose Erbu Zhuyu Tang groups and aspirin group (each n=10). The model of embryo implantation dysfunction was established by using mifepristone. The mice in all groups were orally given corresponding medicinals each day and executed in the afternoon of the 5th d of pregnancy. The morphology of uterus was observed, pregnancy rate and average number of embryo implantation sites were calculated, and mRNA and protein expressions of Beclin-1 and LC3B were detected. Results The color of uterus was ruddy and embryo implantation sites were intumescent and distributed equally in blank group. The color of uterus was pale and slender in model group. The uterus was slender and its color was pink in medicated groups. The number of embryo implantation sites decreased in model group compared with blank group (P<0.001). Compared with model group, the number of embryo implantation sites all increased in high-dose Erbu Zhuyu Tang group and aspirin group (P<0.05). The mRNA and protein expressions of Beclin-1 and LC3B were lower in model group than those in blank group (P<0.05). Compared with model group, the mRNA and protein expressions of Beclin-1 and LC3B increased in low-dose and high-dose Erbu Zhuyu Tang groups and aspirin group (P<0.05). Conclusion Erbu Zhuyu Tang can increase the mRNA and protein expressions of Beclin-1 and LC3B, promote autophagy level of endometrial cells and improve endometrial receptivity and average number of embryo implantation sites.

Key words: Erbu Zhuyu Tang (Decoction of Promoting Fertility with Two Tonifying Medicinals), embryo implantation dysfunction, endometrial receptivity, autophagy, mice

中图分类号: 

  • R285.5