主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (4): 304-309.doi: 10.3969/j.issn.1006-2157.2020.04.007

• 中药药理 • 上一篇    下一篇

祛风宣肺方对咳嗽高敏感性豚鼠神经源性炎症的作用及机制研究

王颖1,史利卿2,马建岭2#,季坤2,李扭扭2,王丽云2,董尚娟2,温绍惠2,罗敬月1   

  1. 1 北京中医药大学 北京 100029;
    2 北京中医药大学东方医院
  • 收稿日期:2019-10-31 出版日期:2020-04-30 发布日期:2020-05-14
  • 通讯作者: 马建岭,男,博士,副主任医师,硕士生导师,研究方向:中医药治疗呼吸系统疾病,Email:ponymjl@sina.com
  • 作者简介:王颖,女,在读博士生
  • 基金资助:
    国家自然科学基金资助项目(No.81373588),北京市自然科学基金资助项目(No.7184224,No.7192115),北京中医药大学东方医院“1166”人才培养项目

Effects and mechanism of Qufeng Xuanfei formula on neurogenic inflammation in guinea pigs with cough hypersensitivity*

Wang Ying1, Shi Liqing2, Ma Jianling2#, Ji Kun2, Li Niuniu2, Wang Liyun2, Dong Shangjuan2, Wen Shaohui2, Luo Jingyue1   

  1. 1 Beijing University of Chinese Medicine,Beijing 100029, China;
    2 Dongfang Hospital, Beijing University of Chinese Medicine,Beijing 100078, China
  • Received:2019-10-31 Online:2020-04-30 Published:2020-05-14
  • Contact: Ma Jianling, A/Chief physician, Master Supervisor. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078. E-mail: ponymjl@sina.com
  • Supported by:
    National Natural Science Foundation of China (No. 81373588); Beijing Municipal Natural Science Foundation (No. 7184224,No. 7192115)

摘要: 目的 探讨祛风宣肺方对咳嗽高敏感性豚鼠神经源性炎症的作用及机制。方法 48只豚鼠随机分为正常组、模型组、辣椒平组及祛风宣肺方组,每组12只。采用环磷酰胺、卵蛋白和氢氧化铝制备咳嗽高敏感豚鼠模型。造模成功后正常组及模型组予生理盐水灌胃;辣椒平组予瞬时受体电位阳离子通道V1(TRPV1)抑制剂辣椒平腹腔注射(1 μmol/kg),每日1次;祛风宣肺方组予祛风宣肺方灌胃(5 g/kg),每日1次。给药7 d后辣椒素雾化测定各组豚鼠的咳嗽次数,肺功能仪检测不同浓度氯化乙酰胆碱激发下豚鼠的气道阻力,HE染色法观察各组豚鼠肺组织的病理变化,RT-PCR法测定各组豚鼠肺组织中TRPV1、P物质(SP)、降钙素基因相关肽(CGRP)的基因表达。结果 与正常组比较,模型组咳嗽次数,气道阻力,肺组织TRPV1、SP、CGRP基因表达增加(P<0.05);与模型组比较,祛风宣肺方组、辣椒平组豚鼠咳嗽次数,气道阻力,肺组织TRPV1、SP、CGRP基因表达降低(P<0.05);祛风宣肺方组肺组织TRPV1、SP、CGRP基因表达低于辣椒平组,差异有统计学意义(P<0.01)。与正常组比较,模型组肺组织结构破坏及炎症反应明显,辣椒平组及祛风宣肺方组肺组织结构较模型组改善,炎症水平降低。结论 祛风宣肺方可降低咳嗽高敏感豚鼠的气道敏感性,其作用机制可能与抑制TRPV1进而减轻气道神经源性炎症有关。

关键词: 祛风宣肺方, 慢性咳嗽, 咳嗽高敏感, 神经源性炎症, 瞬时受体电位阳离子通道V1, 豚鼠

Abstract: Objective To investigate the effects of Qufeng Xuanfei (Wind-expelling Lung-dispersing, QFXF) formula on neurogenic inflammation in guinea pigs with cough hypersensitivity. Methods 48 guinea pigs were randomly divided into the normal group, model group, capsazepine (intraperitoneal injection, 1 μmol/kg ) group and QFXF formula (5 g/kg) group (n=12 in each group). After treated with cyclophosphamide, ovalbumin and aluminium hydroxide to establish the model of cough hypersensitivity, the guinea pigs were orally given saline in the normal group and model group for 7 days. Then the number of cough was counted immediately after using atomized capsaicine stimulation; the airway resistance was measured with spirometer under different concentrations of acetylcholine chloride. Lung pathology was evaluated by using HE staining. The mRNA expression of lung TRPV1, SP, and CGRP was measured by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The number of cough, the airway resistance and the mRNA expression of lung TRPV1, SP, and CGRP increased significantly in the model group compared with the normal group (P<0.05), and decreased significantly in the treatment groups compared with the model group (P<0.05). The mRNA expression of TRPV1, SP, and CGRP decreased significantly in the QFXF formula group compared with capsazepine group (P<0.05); The lung tissue in the model group showed apparent structure destruction and tissue inflammation compared with the normal group and the level of the destruction and inflammation was lower in the treatment groups compared with the model group. Conclusion Qufeng Xuanfei formula could reduce the level of the airway sensitivity in guinea pig models with cough hypersensitivity, and the mechanism may be related to relieving the airway neurogenic inflammation by inhibiting TRPV1 channel.

Key words: Qufeng Xuanfei formula, chronic cough, cough hypersensitivity, neurogenic inflammation, transient receptor potential V1, guinea pigs

中图分类号: 

  • R285.5