主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (5): 393-401.doi: 10.3969/j.issn.1006-2157.2020.05.008

• 中药药理 • 上一篇    下一篇

海藻玉壶汤及拆方对甲状腺肿大大鼠甲状腺

吕艳敏, 修琳琳#, 柳海艳, 张晶璇, 李娜, 张晨, 许皖, 陈丰, 王鑫, 葛超冉, 陈绍红, 钟赣生   

  1. 北京中医药大学中医学院 北京 100029
  • 收稿日期:2019-11-04 发布日期:2020-06-08
  • 通讯作者: #修琳琳,女,博士,讲师,主要研究方向:中药药性理论与应用研究,E-mail:xiulinlin1231@126.com
  • 作者简介:吕艳敏,女,在读硕士生
  • 基金资助:
    *国家自然科学基金资助项目(No.81573630),中央高校基本科研业务费专项资金(2018-JYBZZ-XJSJJ001)

Effect of Haizao Yuhu Decoction and its recipe removing antagonistic medicinal combination on the thyroid hormone level and tissue morphology in rats with goiter*

Lyu Yanmin, Xiu Linlin#, Liu Haiyan, Zhang Jingxuan, Li Na, Zhang Chen, Xu Wan, Chen Feng, Wang Xin, Ge Chaoran, Chen Shaohong, Zhong Gansheng   

  1. Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2019-11-04 Published:2020-06-08
  • Contact: Xiu Linlin, Ph.D., Lecturer. Beijing University of Chinese Medicine, No.11 North Third Ring Road, Chaoyang District, Beijing 100029, China. E-mail: xiulinlin1231@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81573630); Research Project for Fundamental Research in Central Universities (No. 2018-JYBZZ-XJSJ001)Ethical review: Committee of Ethic Review on Medical and Experimental Animals in Beijing University of Chinese Medicine (No. BUCM-4-2018110202-4036)

摘要: 目的 探讨海藻玉壶汤中加减运用反药组合对甲状腺肿大模型大鼠疗效的影响。方法 将大鼠随机分为空白组、模型组、阳性药(优甲乐)组、海藻玉壶汤全方组、海藻玉壶汤去反药组3组(分别为海藻玉壶汤减甘草组、海藻玉壶汤减海藻组、海藻玉壶汤减海藻甘草组),除空白组外,其余各组灌服丙硫氧嘧啶复制甲状腺肿大模型,以优甲乐作为阳性对照药,各给药组分别给予相应药物。检测各组大鼠甲状腺系数,甲状腺激素水平,甲状腺激素的合成、转运、调控等相关指标;光镜下观察大鼠甲状腺组织形态改变,并统计切片细胞核数、滤泡面积。结果 海藻玉壶汤同阳性药一致,表现出对甲状腺肿大模型大鼠甲状腺激素水平的回调作用,且较好地恢复了模型大鼠甲状腺的形态。全方组与各拆方组比较甲状腺激素相关指标水平,其恢复情况为海藻玉壶汤组>海藻玉壶汤减甘草组>海藻玉壶汤减海藻组>海藻玉壶汤减海藻甘草组;与各拆方组比较甲状腺组织病理形态,其改善情况为海藻玉壶汤组>海藻玉壶汤减海藻甘草组>海藻玉壶汤减海藻组>海藻玉壶汤减甘草组。结论 海藻玉壶汤全方组对甲状腺肿大大鼠甲状腺激素水平以及病理形态的改善要优于去掉反药的各拆方组,拆方组中海藻玉壶汤减海藻甘草组疗效最差。

关键词: 海藻玉壶汤, 反药组合, 甲状腺肿, 甲状腺激素, 甲状腺形态

Abstract: Objective To comparative the thyroid hormone level and histopathological changes of the thyroid tissue in PTU-induced goiter rats treated with Haizao Yuhu Decoction and its recipe minus antagonistic medicinal combination, and to explore whether eliminating antagonistic medicinal combination in Haizao Yuhu Decoction can affect the therapeutic effect. Methods The rats were randomly divided into the blank group, model group, positive drug group (euthyrox),Haizao Yuhu Decoction group,Haizao Yuhu Decoction minus Glycyrrhizae group, Haizao Yuhu Decoction minus Sargassum group, and Haizao Yuhu Decoction minus Sargassum and Glycyrrhizae group. The rat model of goiter was induced with propylthiouracil. Each group was fed with the corresponding drug or decoction. The concentration of serum related indicators was detected, including the thyroid coefficient, thyroid hormone level and its synthesis, transport and regulation. Under light microscope, pathological changes of thyroid tissue in rats were observed, and nucleic number and follicular area in sections were counted. Results Like the positive drug, Haizao Yuhu Decoction was consistent with the positive drug of euthyrox in maintaining thyroid functions and recovering the pathological damage of thyroid tissue in PTU-induced goiter rats. Comparing the thyroid hormone related indicators,the degree of recovery was listed: Haizao Yuhu Decoction group>Haizao Yuhu Decoction minus Glycyrrhizae group>Haizao Yuhu Decoction minus Sargassum group>Haizao Yuhu Decoction minus Sargassum and Glycyrrhizae group. Comparing the histopathological changes of the thyroid tissues, the alleviating degree was listed: Haizao Yuhu Decoction group>Haizao Yuhu Decoction minus Sargassum and Glycyrrhizae group>Haizao Yuhu Decoction minus Sargassum group>Haizao Yuhu Decoction minus Glycyrrhizae group. Conclusion The therapeutic effect of Haizao Yuhu Decoction in regulating thyroid hormone level and improving histopathological changes in goiter rats seems to be better than its recipe minus antagonistic medicinal combination, and the Haizao Yuhu Decoction minus Sargassum and Glycyrrhizae tends to have the least effect.

Key words: Haizao Yuhu Decoction, antagonistic medicinal combination, goiters, thyroid hormone, thyroid morphology

中图分类号: 

  • R285.5