主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (6): 492-497.doi: 10.3969/j.issn.1006-2157.2020.06.009

• 中药药理 • 上一篇    下一篇

活血消异方对子宫内膜异位症大鼠卵巢颗粒细胞超微结构的影响*

时光1, 刘永1, 孙伟伟1, 董晓英2, 韩倩3, 鲍美如1,4, 赵瑞华1#   

  1. 1 中国中医科学院广安门医院妇科 北京 100053;
    2 首都医科大学中医药学院;
    3 首都医科大学附属北京妇产医院中医科;
    4 北京中医药大学
  • 收稿日期:2019-10-26 发布日期:2020-07-06
  • 通讯作者: #赵瑞华,女,博士,教授,主任医师,博士生导师,主要研究方向:中医药治疗子宫内膜异位症的临床与基础研究,E-mail:rhzh801@126.com
  • 作者简介:时光,女,在读博士生
  • 基金资助:
    *北京市自然科学基金资助项目(No.7182141)

Effects of Huoxue Xiaoyi Fang on ultrastructure of ovarian granulosa cells in rats with endometriosis*

Shi Guang1, Liu Yong1, Sun Weiwei1, Dong Xiaoying2, Han Qian3, Bao Meiru1,4, Zhao Ruihua1#   

  1. 1 Department of Gynecology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China;
    2 School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069,China;
    3 Department of Traditional Chinese Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
    4 Beijing University of Chinese Medicine, Beijing 100029,China
  • Received:2019-10-26 Published:2020-07-06
  • Contact: Prof. Zhao Ruihua, Ph.D., Doctoral Supervisor. Guang’anmen Hospital, China Academy of Chinese Medical Sciences. No.5 Beixian’ge Street, Xicheng District, Beijing 100053. E-mail: rhzh801@126.com
  • Supported by:
    Beijing Natural Science Foundation (No.7182141)

摘要: 目的 探讨活血消异方对子宫内膜异位症大鼠卵巢颗粒细胞超微结构的影响。方法 应用同种异体子宫内膜移植法建立子宫内膜异位症大鼠模型,将大鼠随机分为空白组、假手术组、模型组、活血消异方组。子宫内膜异位症模型建立后,空白组、假手术组、模型组予蒸馏水,活血消异方组予活血消异方连续灌胃15 d。给药结束后,取各组大鼠卵巢组织行HE染色观察卵泡发育,免疫组化行卵泡刺激素受体(FSHR)多克隆抗体染色定位卵巢颗粒细胞,透射电镜观察各组卵巢颗粒细胞超微结构。结果 子宫内膜异位症模型大鼠原始卵泡、初级卵泡、次级卵泡、成熟卵泡数目均减少,黄体数目明显增多,颗粒细胞排列散乱;卵巢颗粒细胞超微结构严重破坏,甚则出现核染色质边集,线粒体空泡化,内质网脱颗粒等不可逆细胞器损伤。空白组、假手术组、活血消异方组大鼠各级卵泡均发育,卵巢颗粒排列整齐,细胞器结构完整。结论 子宫内膜异位症引起模型大鼠卵巢颗粒细胞线粒体、内质网等细胞器严重破坏,影响卵泡发育;活血消异方通过减轻颗粒细胞超微结构损伤,改善卵泡发育。

关键词: 子宫内膜异位症, 活血消异方, 卵巢颗粒细胞, 超微结构, 大鼠

Abstract: Objective To investigate the effects of Huoxue Xiaoyi Fang (Blood-moving and Endometriosis-removing Formula, HXXYF) on the ultrastructure of ovarian granulosa cells in rats with endometriosis. Methods Rats were randomly divided into blank group, sham-operated group, model group, and HXXYF group. The latter two groups were formed in the following way. First, rat models with endometriosis were established by allogeneic endometrial transplantation from donors to recipients and recipients were then randomly divided into model group and HXXYF group. After model establishment, while blank group, sham-operated group, and model groupwere given distilled water, HXXYF group was given HXXYF by gavage for 15 consecutive days. Then ovarian tissues of rats in different groups were taken for HE staining to observe follicular development, FSHR polyclonal antibody immunohistochemical staining to locate ovarian granulosa cells, and transmission electron microscopy to observe ultrastructure of ovarian granulosa cells. Results In model group, the numbers of primordial follicles, primary follicles, secondary follicles, and mature follicles were decreased, the numbers of corpus luteum were increased significantly, and granulosa cells were poorly arranged. In addition, ultrastructure of ovarian granulosa cells was severely damaged, and even demonstrated irreversible organelle damage, such as nucleus chromatin margination, mitochondrial vacuolation, and endoplasmic reticulum degranulation.In blank group, sham-operated group and HXXYF group, follicles were all developed at different levels, ovarian granulosa cells were arranged in a regular way, and organelle structure was complete. Conclusion Endometriosis causes serious damage to organelles of ovarian granulosa cells in rats like mitochondrion and endoplasmic reticulum, and affects follicular development. HXXYF can improve follicular development by reducing ultrastructural damage to granulosa cells in rats with endometriosis.

Key words: endometriosis, Huoxue Xiaoyi Fang, granulosa cell, ultrastructure, rats

中图分类号: 

  • R285.5