主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (8): 680-688.doi: 10.3969/j.issn.1006-2157.2020.08.011

• 科技之窗 • 上一篇    下一篇

基于磁共振成像研究侯氏黑散对脑缺血大鼠神经血管功能恢复的作用*

程宏发, 王璇, 张雅文, 赵晖, 王蕾, 邹海艳, 张秋霞#   

  1. 首都医科大学中医药学院 中医络病研究北京市重点实验室 北京 100069
  • 收稿日期:2020-03-28 出版日期:2020-08-30 发布日期:2020-08-27
  • 通讯作者: # 张秋霞,女,博士,教授,博士生导师,主要研究方向:中医药防治脑病,E-mail:zqx26@163.com
  • 作者简介:程宏发,男,在读硕士生
  • 基金资助:
    * 国家自然科学基金面上项目(No.81373526,No.81873224),北京市自然科学基金项目(No.7102014,No.7122018),北京市属高等学校高层次人才引进与培养计划-长城学者基金支持项目(No.CIT &TCD20140329)

Effects of Houshiheisan Formula on the recovery of neurovascular function in rats with cerebral ischemia based on MRI*

Cheng Hongfa, Wang Xuan, Zhang Yawen, Zhao Hui, Wang Lei, Zou Haiyan, Zhang Qiuxia#   

  1. School of Traditional Chinese Medicine, Capital Medical University, Beijing Key Laboratory of TCM Collateral Disease Theory Research, Beijing 100069, China
  • Received:2020-03-28 Online:2020-08-30 Published:2020-08-27
  • Contact: Prof. Zhang Qiuxia, M.D., School of Traditional Chinese Medicine, Capital Medical University, No.10 Youanmen west, Fengtai District, Beijing 100069. E-mail: zqx26@163.com
  • Supported by:
    National Natural Science Foundation of China (No. 81373526, No. 81873224), Natural Science Foundation of Beijing (No. 7102014, No. 7122018)

摘要: 目的 利用磁共振成像技术研究侯氏黑散对脑缺血大鼠神经血管功能的影响。方法 采用大脑中动脉线栓法建立大鼠永久性脑缺血模型。雄性SD大鼠随机分为假手术组、模型组、侯氏黑散低剂量组(5.25 g/kg)组、侯氏黑散高剂量组(10.50 g/kg)组和银杏叶提取物组(28 mg/kg)。神经功能评分评价各组大鼠神经功能缺损程度;T2加权成像和T2弛豫成像测量脑缺血大鼠脑梗死体积和脑组织损伤程度;动脉自旋标记灌注加权成像和磁共振血管成像评价脑缺血大鼠颅内血管的血流动力学的改变。结果 与模型组相比,侯氏黑散高剂量组与银杏叶提取物组神经功能评分在脑缺血后第5、7天降低(P<0.05)。与模型组相比,各给药组脑梗死体积缩小,脑白质和灰质损伤减轻(P<0.05),缺血半球脑血流量和血管信号强度增加(P<0.05)。与假手术组相比,模型组缺血半球脑血流量和血管信号强度降低(P<0.01)。结论 侯氏黑散可以减轻脑缺血大鼠脑损伤和改善颅内血流动力学,起到对神经血管功能的保护和恢复作用。

关键词: 脑缺血, 侯氏黑散, 磁共振成像, 灌注加权成像, 磁共振血管成像, 神经血管单元, 大鼠

Abstract: Objective To non-invasively assess the therapeutic effect of Houshiheisan (HSHS) Formula on the recovery of neurovascular function after cerebral ischemia in rats with application of magnetic resonance imaging (MRI). Methods Focal cerebral ischemia model was induced by permanent middle cerebral artery occlusion (pMCAO) in male sprague-Dawley (SD) rats. Then the rats were randomly divided into 5 groups: sham group, model group, HSHS low-dose group (5.25 g/kg), HSHS high-dose group (10.50 g/kg) and Ginkgo biloba extract group (28 mg/kg). Nurological score was assessed after surgery to evaluate the degree of nervous function defects. T2 weighted imaging (T2WI) and T2 relaxation imaging were used to measure the volume of cerebral infarction and the degree of brain tissue damage in rats with cerebral ischemia. Arterial spin labeled perfusion weighted imaging(ASL-PWI)and magnetic resonance angiography (MRA) were used to evaluate the hemodynamic changes of intracranial vessels. Results Compared with the model group, HSHS high-dose group and Ginkgo biloba extract group decrease neurological function score on the 5th and 7th day (P<0.05). Compared with the model group, the infarct volume reduced and the damage of white matter and gray matter was decreased, and the cerebral blood flow and signal intensity of ischemic hemisphere increased in all treatment groups (P<0.05). Compared with the sham group, the cerebral blood flow and signal intensity of ischemic hemisphere decreased in the model group (P<0.01). Conclusion HSHS seems to alleviate brain injury and improve the intracranial hemodynamics, and promote the recovery of neurovascular function.

Key words: cerebral ischemia, Houshiheisan (HSHS) Formula, Magnetic Resonance Imaging (MRI), perfusion-weighted imaging, magnetic resonance angiography, neurovascular unit, rats

中图分类号: 

  • R285.5