主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (9): 754-761.doi: 10.3969/j.issn.1006-2157.2020.09.009

• 中药药理 • 上一篇    下一篇

蒌芩止嗽优化方对铜绿假单胞菌感染机体免疫失衡的调控*

李杰1, 吴童1, 于子洋1, 刘丽婷1, 黄明明1, 赵翠云1, 孟子霞1, 彭桂英2#   

  1. 1 北京中医药大学东直门医院 北京 100700;
    2 北京中医药大学生命科学学院
  • 收稿日期:2020-03-16 发布日期:2020-09-29
  • 通讯作者: #彭桂英,女,博士,副教授,硕士生导师,主要研究方向:炎症性疾病机制及中医药免疫调节作用研究,E-mail:pengguiying@bucm.edu.cn
  • 作者简介:李杰,女,博士,副教授,主任医师
  • 基金资助:
    *国家自然科学基金青年基金项目(No.81202681),北京中医药大学2019年度基本科研业务费创新科研团队项目(No.2019-JYB-TD014)

Effects of Louqin Zhisou Youhua Fang on improving antibiotic permeability through Pseudomonas aeruginosa biofilm by correcting inflammatory immune imbalance*

Li Jie1, Wu Tong1, Yu Ziyang1, Liu Liting1, Huang Mingming1, Zhao Cuiyun1, Meng Zixia1, Peng Guiying2#   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700 China;
    2 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 102488,China
  • Received:2020-03-16 Published:2020-09-29
  • Contact: Associate Prof. Peng Guiying, Doctor of Science, Master’s Supervisor. Beijing University of Chinese Medicine. Intersection of Yangguang Nandajie Street and Baiyang Donglu Road, Liangxiang Campus of Beijing University of Chinese Medicine, Liangxiang Higher Education Park, Fangshan District, Beijing 102488. E-mail: Pengguiying@bucm.edu.cn
  • Supported by:
    National Natural Science Foundation of China for Young Scientists (No. 81202681)

摘要: 目的 探究蒌芩止嗽优化方对机体铜绿假单胞菌(PA)感染的免疫保护作用。方法 体外实验观察蒌芩止嗽优化方对左氧氟沙星透过PA成熟生物被膜(BF)的影响。体内实验观察其对PA感染大鼠肺部组织TNF-α、IFN-γ的影响,探究蒌芩止嗽优化方对炎症机体免疫失衡的调控。结果 体外,蒌芩止嗽优化方与氨溴索均可以协助左氧氟沙星透过PA成熟生物被膜。体内,西药组与中药组均可促进肺部细菌的清除,并且有助于减轻肺组织的病理损伤;中药组与模型组比较肺部的TNF-α水平明显降低(P< 0.001),IFN-γ水平显著提高(P< 0.001);西药组与模型组比较肺部的TNF-α水平明显降低(P< 0.001),IFN-γ水平虽轻微提高,但差异无统计学意义;中药组与西药组比较肺部的TNF-α水平明显降低(P< 0.001),IFN-γ水平显著提高(P< 0.001)。结论 蒌芩止嗽优化方体外可协助抗生素透过成熟生物被膜,其机制可能是通过纠正PA感染大鼠体内炎症免疫失衡,一方面加快了肺部细菌的清除及减轻肺部病理损伤,从而减轻机体炎症反应;另一方面增强机体对PA 生物被膜感染的抵抗力,从而间接达到清除细菌的作用,它与氨溴索比较的优势可能亦在于可以提高机体免疫力,其深层作用机制尚有待进一步深入探讨。

关键词: 蒌芩止嗽优化方, 炎症免疫失衡, 抗生素, 铜绿假单胞菌, 生物被膜, 大鼠

Abstract: Objective To explore the immune protective effects of Louqin Zhisou Youhua Fang (Snakegourd-fruit Scutellaria-root Cough-stopping Formula with Modifications, LQZSYHF) on infection with Pseudomonas aeruginosa. Methods The effects of LQZSYHF on improving the penetration of levofloxacin through the PA mature biofilm (BF) were studied via in vitro experiment. Its effects on regulating inflammatory immune imbalance were explored through observing the levels of TNF-α and IFN-γ in the lung tissues of PA-infected rats via in vivo experiment. Results In vitro, both LQZSYHF and ambroxol assisted levofloxacin in penetrating PA mature biofilms. In vivo, both the Western drug and Chinese herbal medicine significantly promoted the elimination of lung bacteria and reduced the pathological damage to lung tissue. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the Chinese herbal medicine (CHM) group compared with the model group (P<0.001). TNF-α level in lung tissue was greatly significantly reduced (P<0.001) and IFN-γ level was slightly increased without statistical significance in the Western drug (WG) group compared with the model group. TNF-α level in lung tissue was significantly reduced and IFN-γ level was greatly increased in the CHM group compared with WD group (P <0.001). Conclusion LQZSYHF can help antibiotics pass through mature biofilms in vitro. Its mechanism might be that it can correct the immune imbalance in PA-infected rats, by which it accelerates the elimination of lung bacteria and reduces lung pathological damage, thereby reducing inflammatory response, and it enhances body resistance against PA biofilm infection, thereby indirectly eliminating bacteria. Compared with ambroxol, its advantages may lie in improving immunity, and its underlying mechanism warrants further study.

Key words: Louqin Zhisou Youhua Fang, inflammatory immune imbalance, antibiotic, Pseudomonas aeruginosa, biofilm, rats

中图分类号: 

  • R285.5