主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (11): 935-940.doi: 10.3969/j.issn.1006-2157.2020.11.008

• 中药药理 • 上一篇    下一篇

参附黄配方对脓毒症小鼠认知功能和突触可塑性的影响*

王雪蕊, 郭玉红, 赵京霞, 徐霄龙, 刘清泉#   

  1. 首都医科大学附属北京中医医院 北京 100010
  • 收稿日期:2020-05-18 出版日期:2020-11-30 发布日期:2020-12-08
  • 通讯作者: #刘清泉,男,主任医师,博士生导师,主要研究方向:中西医结合治疗急危重症,E-mail:liuqingquan_2003@126.com
  • 作者简介:王雪蕊,女,硕士,研究实习员
  • 基金资助:
    *国家自然科学基金面上项目(No.81673934,No.81973608),国家自然科学基金青年项目(No.82004292),国家科技重大专项(No.2017XZ10305501)

Effects of Shenfuhuang Formula on cognitive function and synaptic plasticity in septic mice*

Wang Xuerui, Guo Yuhong, Zhao Jingxia, Xu Xiaolong, Liu Qingquan#   

  1. Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China
  • Received:2020-05-18 Online:2020-11-30 Published:2020-12-08
  • Contact: Liu Qingquan,Chief Physician,Doctoral Supervisor.Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,No.23 Meishuguan Bavk Street,Dongcheng District,Beijing 100010.E-mail:liuqingquan_2003@126.com

摘要: 目的 探讨参附黄配方对脓毒症小鼠空间学习记忆、焦虑状态及海马CA1区突触可塑性的影响。方法 C57BL/6小鼠随机分为对照组、脓毒症组和参附黄组。脓毒症组和参附黄组采取盲肠结扎穿孔术(CLP)制备脓毒症模型。参附黄组术后给予每日2次的2.5g/kg参附黄配方灌胃,灌胃体积0.5 mL;脓毒症组和对照组给予每日2次的0.5 mL纯净水灌胃。采用Morris水迷宫实验评价小鼠空间学习记忆能力的变化,采用旷场实验评价小鼠探索能力和焦虑状态的变化,采用在体神经电生理技术记录海马CA1 区长时程增强效应。结果 与对照组比较,脓毒症组Morris水迷宫逃避潜伏期和目标象限时间显著降低(P<0.05),旷场实验跨格次数、竖起次数和中央区停留时间显著降低(P<0.05),海马CA1 区场兴奋性突触后电位斜率显著降低(P<0.05);与脓毒症组相比较,参附黄组逃避潜伏期和目标象限时间显著升高(P<0.05),跨格次数、竖起次数和中央区停留时间显著延长(P<0.05),兴奋性突触后电位斜率显著升高(P<0.05)。结论 参附黄配方对CLP小鼠认知行为能力具有一定改善作用,其机制可能与改善海马区突触可塑性有关。

关键词: 脓毒症, 参附黄, 认知功能, 突触可塑性, 小鼠

Abstract: Objective To investigate the effect of Shenfuhuang Formula on spatial learning and memory,anxiety state,and hippocampal CA1 synaptic plasticity in sepsis mice.Methods C57BL/6 mice were randomly divided into control group,model group and Shenfuhuang group.The sepsis model was established with cecal ligation and perforation (CLP) in the latter two groups.The Shenfuhuang group was gavaged with 2.5 g/kg Shenfuhuang Decoction,twice daily,with a total volume of 0.5 mL;the model group and the control group were given 0.5 mL normal saline twice daily.Morris water maze test was used to evaluate the spatial learning and memory ability.The open field test was used to evaluate the exploration ability and anxiety state.The long-term potentiation of hippocampal CA1 area was also recorded in vivo by neurophysiological technology.Results Compared with the control group,in model group the escape latency and the time spent in target quadrant of Morris water maze were significantly reduced (P<0.05);the number of crossing,rearing and center duration of the open field test were significantly reduced (P<0.05);and the slope of the field post-synaptic potential were significantly reduced (P<0.05).Compared with the model group,in Shenfuhuang group the escape latency and the time spent in target quadrant were significantly increased (P<0.05);the number of crossing,rearing and center duration of the open field test were significantly increased (P<0.05);and the slope of the field post-synaptic potential were significantly increased (P<0.05).Conclusion Shenfuhuang Formula seems to improve the cognitive impairment and behavioral ability of CLP mice to a certain degree,and its mechanism may be related to the improvement of synaptic plasticity in the hippocampus.

Key words: sepsis, Shenfuhuang Formula, cognitive function, synaptic plasticity, mouse

中图分类号: 

  • R285.5