主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (12): 973-979.doi: 10.3969/j.issn.1006-2157.2020.12.001

• 专家述评 •    下一篇

慢性胃炎“炎癌转化”病证结合大鼠模型建立中的难点问题及应对策略*

丁霞1, 苏泽琦2, 沈洪3, 张平4   

  1. 1 北京中医药大学中医学院 北京 100029;
    2 北京中医药大学北京中医药研究院;
    3 江苏省中医院;
    4 中国中医科学院望京医院
  • 收稿日期:2020-08-27 出版日期:2020-12-30 发布日期:2021-01-05
  • 作者简介:丁霞,女,博士,教授,博士生导师,主要研究方向:消化系统疾病的中西医结合基础与临床研究,E-mail: dingx@bucm.edu.cn
  • 基金资助:
    *国家自然科学基金项目(No. 81703931,No.81630080),北京中医药大学杰出青年人才项目(No. BUCM-2019-JCRC001)

Challenges and solutions in the establishment of rat models of gastritis deteriorating into gastric carcinoma*

Ding Xia1, Su Zeqi2, Shen Hong3, Zhang Ping4   

  1. 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Beijing Research Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    3 Jiangsu Province Hospital of Chinese Medicine, Jiangsu 210029, China;
    4 Wang Jing Hospital of China Academy of Chinese Medical Sciences, Beijing 100102, China
  • Received:2020-08-27 Online:2020-12-30 Published:2021-01-05
  • Contact: Prof.Ding Xia, Ph.D.,Doctoral Supervisor. School of Chinese Medicine,Beijing University of Chinese Medicine. No.11 Beisanhuan Donglu Road,Chaoyang District,Beijing 100029. E-mail: dingx@bucm.edu.cn
  • Supported by:
    National Natural Science Foundation of China (No. 81703931, No. 81630080)

摘要: 慢性胃炎“炎癌转化”是胃癌发生的主要途径,在此阶段进行积极应对,对于降低胃癌发生率具有重要意义。然而由于目前在造模方法、模型评价、模型构建思路、模型证候判定等方面存在诸多瓶颈问题,制约了该领域基础及应用研究的开展。就此,基于本团队近5年的研究成果,结合国内外研究进展,围绕慢性胃炎“炎癌转化”病证结合动物模型建立中的难点,逐一进行分析并提出应对策略;同时模拟临床多因素致病的特点,提供了2种以N-甲基-N'-硝基-亚硝基胍(MNNG)为主要造模因素的大鼠模型制备方案,相较于现有模型,具有符合临床特征、成模率高、稳定性好等优势,是该领域动物实验研究的有力工具。

关键词: 慢性胃炎, 癌前病变, 炎癌转化, 动物模型, 病证结合

Abstract: Since “deterioration of chronic gastritis into gastric cancer” is the main cause of gastric cancer, active prevention and treatment of such deterioration is of great significance for reducing the incidence of gastric cancer. However, many bottlenecks in model establishment have restricted the development of basic research and application in this field, e.g. the selection of model factors, model preparation methods, model evaluation at different pathological stages, the way of combining WM disease and TCM pattern in model establishment, and model pattern determination. Based on the research findings of the present team in the last 5 years as well as global research progress, problems in the establishment of the animal models of gastritis deteriorating into gastric carcinoma with consideration of both WM disease and TCM pattern were analyzed one by one and possible solutions were proposed. We simulated the clinical features of the multifactorial disease and provided two rat models with MNNG as the main factor. Compared with the existing models, they are proved more in line with clinical characteristics of the disease and yield higher success rates for model establishment and better stability by multiple experiments. They may become powerful tools for animal research in this field.

Key words: chronic gastritis, precancerous lesion, deterioration of gastritis into gastric cancer, animal model, combination of disease and pattern

中图分类号: 

  • R259.733