主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2021, Vol. 44 ›› Issue (1): 39-44.doi: 10.3969/j.issn.1006-2157.2021.01.007

• 中药药理 • 上一篇    下一篇

通咽喷雾方对吞咽障碍大鼠舌下神经核运动相关递质的影响*

简郭血骄, 李春蕊, 胡素芹, 郭健#   

  1. 北京中医药大学中医学院 北京 102488
  • 收稿日期:2020-07-22 出版日期:2021-01-30 发布日期:2021-01-18
  • 通讯作者: #郭健,女,博士,教授,博士生导师,主要研究方向:中医药物质基础的研究,E-mail: guojian@bucm.edu.cn
  • 作者简介:简郭血骄,男,硕士
  • 基金资助:
    *国家自然科学基金资助项目(No.81473623),北京中医药大学科研纵向发展基金(No.2019ZXFZJJ021)

Effects of Tongyan spray Formula on motility related neurotransmitters in hypoglossal nuclei of rats with dysphagia*

Jian Guoxuejiao, Li Chunrui, Hu Suqin, Guo Jian#   

  1. School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China
  • Received:2020-07-22 Online:2021-01-30 Published:2021-01-18
  • Contact: Prof.Guo Jian,Ph.D.,Doctoral Supervisor.School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Northeast Corner of the Iwtersction of Yangguang South Street and Baiyang East Road,Fangshan District,Beijing 102488.E-mail:guojian323@sina.com.
  • Supported by:
    National Natural Science Foundation of China(No. 81473623);Science development Foundation of Beijing University of Chinese Medicine(2019ZXFZJJ021)

摘要: 目的 探讨通咽喷雾方改善吞咽障碍的机制。方法 成年雄性Wistar大鼠随机分为假手术组、模型组和通咽喷雾方组。假手术组仅分离舌下神经,模型组和通咽喷雾方组分离舌下神经后用止血钳钳夹压榨损伤10 s造模。药物组造模后第2天开始舌滴通咽喷雾方(0.3 mL/次,1次/d),假手术组和模型组舌滴同等剂量生理盐水。给药7 d后取大鼠延髓定位舌下神经核,采用尼氏染色法检测尼氏体(Nissl body),免疫组织化学法检测运动相关神经递质五羟色胺(5-HT)、乙酰胆碱酯酶(AChE)、p38和神经元型一氧化氮合酶(nNOS)的变化。结果 与假手术组相比,模型组尼氏体减少,5-HT、AChE含量均降低,P38和nNOS含量均升高(P<0.05)。给药后,通咽喷雾方组的尼氏体、5-HT和AChE含量均升高(P<0.05),而P38和nNOS含量均降低(P<0.05)。假手术组和通咽喷雾方组比较各神经递质的表达差异亦有统计学意义。结论 通咽喷雾方改善舌下神经钳夹损伤造成的吞咽障碍机制与抑制p38和NO表达、上调兴奋性递质5-HT和ACh水平有关。

关键词: 通咽喷雾方, 吞咽障碍, 舌下神经损伤, 舌下神经核, 大鼠

Abstract: Objective To explore the mechanism of relieving dysphagia by using Tongyan spray formula.Methods Adult Wistar male rats were randomly divided into sham operation group,model group and Tongyan spray formula group.Rats in the sham operation group were only Operated to isolate hypoglossal nerve,and rats in the model group and the Tongyan spray formula group were Operated to isolate hypoglossal nerve and then the vulnus was compressed with hemostatic forceps for 10s to make a model.From the second day after modeling,Tongyan spray formula group was given drugs on the tongue (0.3 mL per time,once a day),and the sham operation group and model group were given the same dose of saline.After administration for 7 days,rat medulla oblongata tissue was collected,Nissl body was detected with Nissl staining,and serotonin (5-HT),Acetylcholinesterase (AChE),p38,Neuronal nitric oxide synthase (nNOS) were detected by using immunohistochemical method.Results Compared with the sham operation group,the expressions of Nissl body,5-HT and AChE were decreased,and p38 and nNOS were increased (P<0.05) in the model group,which referred to the success of modeling.After administration,the expressions of Nissl body,5-HT and AChE were increased (P<0.05),but p38 and nNOS were decreased (P<0.05).There was also a significant difference in the expression of neurotransmitters between the sham operation and Tongyan spray formula group.Conclusion Tongyan spray may relieve the dysphagia due to hypoglossal nerve clamp injury,and its possible mechanism seems to be related to the inhibition of p38 and NO and the increasing of 5-HT and ACh.

Key words: Tongyan spray formula, dysphagia, hypoglossal nerve injury, hypoglossal nuclei, rats

中图分类号: 

  • R285.5