主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2021, Vol. 44 ›› Issue (3): 245-251.doi: 10.3969/j.issn.1006-2157.2021.03.009

• 科技之窗 • 上一篇    下一篇

病证结合脾肾阳虚型IBS-D大鼠模型的建立与评价*

苏晓兰1, 刘倩1, 韩博宇2, 张涛1 , 毛心勇1 , 国嵩1 , 独思静1, 杨洋1, 王欣1, 魏玮1#   

  1. 1 中国中医科学院望京医院脾胃病科 功能性胃肠病中医诊治北京市重点实验室 北京 100102;
    2 北京市隆福医院中医科
  • 收稿日期:2020-11-15 出版日期:2021-03-30 发布日期:2021-03-30
  • 通讯作者: #魏玮,男,博士,主任医师,教授,博士生导师,主要研究方向:中医药防治胃肠疾病,E-mail:sxxtyy@sina.com
  • 作者简介:苏晓兰,女,博士,副主任医师,硕士生导师
  • 基金资助:
    *中央级公益性科研院所基本科研业务费专项资金项目(No.ZZ13-YQ-035),国家自然科学基金项目(No.81774066,No.81973638,No.81820108033),国家重大疑难病中西医协作试点项目

Establishment and evaluation of disease-pattern rat model with IBS-D and spleen-kidney yang deficiency*

Su Xiaolan1, Liu Qian1, Han Boyu2, Zhang Tao1, Mao Xinyong1, Guo Song1, Du Sijing1, Yang Yang1, Wang Xin1, Wei Wei1#   

  1. 1 Department of Spleen and Stomach Diseases, Wang Jing Hospital of China Academy of Chinese Medical Sciences; Beijing Key Laboratory of TCM Diagnosis and Treatment of Functional Gastrointestinal Disorders, Beijing 100102, China;
    2 Department of Chinese Medicine, Beijing Longfu Hospital, Beijing 100010, China
  • Received:2020-11-15 Online:2021-03-30 Published:2021-03-30
  • Contact: Prof. Wei Wei, M.D., Chief Physician, Doctoral Supervisor. Wang Jing Hospital of China Academy of Chinese Medical Sciences. No. 6 Zhonghuan Nan Lu, Chaoyang District, Beijing 100102. E-mail:sxxtyy@sina.com
  • Supported by:
    National Natural Science Foundation of China (No.81774066,No.81973638,No.81820108033), National Pilot Project of Cooperation between Chinese and Western Medicine for Major and Refractory Diseases Ethical review: Animal Ethics Committee of Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences(No.02019-11-14-1)

摘要: 目的 建立并评价病证结合脾肾阳虚型腹泻型肠易激综合征(Diarrhea-predominant irritable bowel syndrome,IBS-D)大鼠模型。方法 72只雄性SD乳鼠随机选取18只作为正常组,余乳鼠进行母子分离21 d构建IBS-D疾病模型;母子分离结束后适应性喂养1周,54只大鼠随机分为高、中、低剂量组,并分设番泻叶灌胃 2、4、6 周组,每组6只。除正常组外,其余各组均叠加高、中、低不同剂量番泻叶灌胃构建脾肾阳虚IBS-D大鼠中医证候模型。在番泻叶灌胃第2、4、6周,分别观察各组大鼠宏观体征、内脏高敏感性、生物学指标和组织病理学变化,进行脾肾阳虚病证结合的模型评价。结果 番泻叶灌胃6周后,高剂量组与正常组比,大鼠进食减少、饮水量增加、肛温降低、握力降低、负重游泳时间降低、粪便粒数增加,大鼠内脏敏感性增加,血清D-木糖、血清促肾上腺皮质激素(ACTH)、血清肾上腺皮质酮(CORT)降低,差异均有统计学意义(P<0.05)。各组大鼠肠道黏膜病理组织学表现均未见明显异常。结论 母子分离叠加番泻叶灌胃可构建病证结合脾肾阳虚型IBS-D大鼠模型,母子分离叠加高剂量番泻叶灌胃6周是构建该病证结合模型较为理想的方法。

关键词: 腹泻型肠易激综合征, 脾肾阳虚, 病证结合, 大鼠

Abstract: Objective To establish and evaluate a rat model with both a Western medicine disease, i.e. diarrhea-predominant irritable bowel syndrome (IBS-D) and a Chinese medicine pattern, i.e. spleen-kidney yang deficiency. Methods Eighteen suckling SD rats were randomly selected from 72 male suckling SD rats as the normal group, and the remaining 54 rats were separated from their mothers for 21 days to construct the IBS-D disease model. After the mother-child separation, the 54 rats were fed regularly for 1 week in adaptation. Then, they were randomly divided into high-dose, mid-dose and low- dose Fanxieye (Senna Leaf, Folium Senae) groups, 6 rats in each group. Each group were further divided into three groups and were given Fanxieye by gavage for 2,4 and 6 weeks respectively to establish the rat model with IBS-D and spleen-kidney yang deficiency. The macroscopic signs, visceral hypersensitivity, biological parameters and histopathological changes of rats in different groups were collected and analyzed to evaluate the model at the 2nd, 4th and 6th week since intragastric administration of Fanxieye. Results After 6 weeks of gavage with Fanxieye, compared with the normal group, rats in the high-dose Fanxieye group ate less, drank more, and had lower anal temperature, reduced grip strength, shorter weight-bearing swimming time, increased number of pellet-like feces, increased visceral sensitivity, and reduced levels of serum D-xylose, adrenocorticotropic hormone (ACTH) and serum cortisone (CORT) , all with statistical significance(P<0.05). There were no obvious histopathological changes in intestinal mucosa in each group. Conclusion The rat model with IBS-D and spleen-kidney yang deficiency can be constructed by mother-child separation superimposed with Fanxieye by gavage. Specifically, mother-child separation combined with high-dose Fanxieye by gavage for 6 weeks is an ideal method to establish such model.

Key words: diarrhea-predominant irritable bowel syndrome / IBS-D, spleen-kidney yang deficiency, rat model, combination of disease and pattern

中图分类号: 

  • R285.5