主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2021, Vol. 44 ›› Issue (3): 252-258.doi: 10.3969/j.issn.1006-2157.2021.03.010

• 中药药理 • 上一篇    下一篇

引经药配伍对血府逐瘀汤预防非酒精性脂肪肝效应机制的影响*

蔡飘1, 宋丽1, 刘嘉欣1, 唐宋琪2, 黄巍1#   

  1. 1 成都中医药大学基础医学院 成都 611137;
    2 海南医学院中医学院
  • 收稿日期:2020-10-23 出版日期:2021-03-30 发布日期:2021-03-30
  • 通讯作者: #黄巍,女,博士,教授,硕士生导师,主要研究方向:方剂配伍理论研究,E-mail:gracehw@126.com
  • 作者简介:蔡飘,女,在读硕士生
  • 基金资助:
    *国家自然科学基金项目(No.81673879)

Influence of envoy herbs on the effect mechanism of Xuefu Zhuyu Tang in treating NAFLD*

Cai Piao1, Song Li1, Liu Jiaxin1, Tang Songqi2, Huang Wei1#   

  1. 1 School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Sichuan 611137, China;
    2 School of Traditional Chinese Medicine, Hainan Medical University, Hainan 571199, China
  • Received:2020-10-23 Online:2021-03-30 Published:2021-03-30
  • Contact: Associate Prof. Huang Wei, Ph.D., Master's Supervisor. Chengdu University of Traditional Chinese Medicine. No. 1166 Liutai Avenue, Wenjiang District, Chengdu 611137. E-mail: gracehw@126.com
  • Supported by:
    National Natural Science Foundation of China (No. 81673879) Ethical review: Ethics Committee of Affiliated Hospital of Chengdu University of Traditional Chinese Medicine (No.2016010221)

摘要: 目的 探讨引经药配伍对血府逐瘀汤预防非酒精性脂肪肝病(NAFLD)的效应机制。方法 采用腹腔注射亚临床剂量脂多糖(LPS)(4 ng/kg)联合高脂饲料喂养建立C57BL/6J ApoE-/-小鼠NAFLD模型,造模期间分别给予血府逐瘀汤、血府逐瘀汤减牛膝方、血府逐瘀汤减牛膝-桔梗方灌胃干预28 d。流式细胞检测全血CD14+CD11b+Ly6G+细胞数量,免疫荧光观测肝组织中性粒细胞浸润情况,酶联免疫吸附法(ELISA)检测CXC趋化因子配体1(CXCL1)、CXC趋化因子配体2(CXCL2)及肝组织髓过氧化物酶(MPO)、CXCL2含量。结果 与模型组相比,血府逐瘀汤组可降低小鼠外周血CD14+CD11b+Ly6G+细胞比例(P<0.05),其作用强度优于血府逐瘀汤减牛膝-桔梗组(P<0.05);血府逐瘀汤组减少中性粒细胞肝组织浸润的作用最为明显;血府逐瘀汤组、血府逐瘀汤减牛膝组、血府逐瘀汤减牛膝-桔梗组可减少外周血CXCL1、CXCL2和肝组织MPO、CXCL2含量(P<0.05),血府逐瘀汤组降低外周血CXCL2和肝组织MPO、CXCL2的作用优于血府逐瘀汤减牛膝-桔梗组(P<0.05)。结论 血府逐瘀汤对C57BL/6J ApoE-/-小鼠NAFLD模型非酒精性脂肪肝形成具有预防作用,其效应机制与中性粒细胞趋化及肝组织内的激活密切相关,血府逐瘀汤中引经药的配伍可显著影响其效应机制。

关键词: 血府逐瘀汤, 非酒精性脂肪肝, 引经药配伍, 小鼠

Abstract: Objective To explore the influence of envoy herbs on the effect mechanism of Xuefu Zhuyu Tang (Blood Stasis Expelling Decoction, XFZYT) in the treatment of NAFLD. Methods C57BL/6J ApoE-/- mice models of NAFLD were established by intraperitoneal injection of subclinical lipopolysaccharide (LPS) (4ng/kg) combined with high-fat diet. During the establishment of the models, XFZYT, XFZYT minus the envoy herbs Niuxi (Two-toothed Achyranthes Root, Radix Achyranthis Bidentatae) and Jiegeng (Platycodon Root, Radix Platycodonis) (XFZYT-NJ) , and XFZYT minus Niuxi (XFZYT-N), were given by gavage to different groups of mice respectively for 28 days. Flow cytometry was used to detect the number of CD14+CD11b+Ly6G+ cells in whole blood. Immunofluorescence (IF) was used to observe neutrophil infiltration. Enzyme-linked immunosorbent assay (ELISA) was used to detect plasma keratin C-X-C motif chemokine ligand 1 (CXCL1) and macrophage inflammatory protein-2 also known as C-X-C motif chemokine ligand 2 (CXCL2), and myeloperoxidase (MPO) and CXCL2 content in liver tissue. Results Compared with the model group, the proportion of CD14+CD11b+Ly6G+ cells in the peripheral blood of mice in the XFZYT group was reduced (P<0.05). Such reduction was greater in the XFZYT group than in the XFZYT-NJ(P<0.05) group. XFZYT had the most significant effect of reducing neutrophil infiltration in liver tissue, compared with its variant formulas. While both XFZYT and its variant formulas reduced CXCL1 and CXCL2 in peripheral blood and MPO and CXCL2 in liver tissue (P<0.05), the former demonstrated greater reduction in CXCL2 in peripheral blood and MPO and CXCL2 in liver tissue than XFZYT-NJ(P<0.05). Conclusion XFZYT could prevent the formation of nonalcoholic fatty liver in C57BL/6J ApoE-/- mice. Its mechanism may be closely related to chemotaxis and activation of neutrophils in liver tissues. The envoy herbs could significant affect such mechanism.

Key words: Xuefu Zhuyu Tang, NAFLD, envoy herb, mouse

中图分类号: 

  • R285.5