主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2021, Vol. 44 ›› Issue (6): 519-526.doi: 10.3969/j.issn.1006-2157.2021.06.006

• 科技之窗 • 上一篇    下一篇

对Ghrelin/GHSR-1a/GABA通路关键因子在胃食管反流病肝郁证模型中表达水平的研究*

陈冬雪1, 钱占红1, 夏聪媛2, 马茹3, 徐愿2, 张丽颖4#   

  1. 1 内蒙古医科大学中医学院 内蒙古 010110;
    2 中日友好医院中医风湿病科 药学部;
    3 北京中医药大学;
    4 中国中医科学院西苑医院脾胃病科
  • 收稿日期:2021-01-03 出版日期:2021-06-30 发布日期:2021-06-25
  • 通讯作者: #张丽颖,女,博士,主治医师,主要研究方向:消化系统疾病的中医临床与基础研究,E-mail:zhangliying2000@163.com
  • 作者简介:陈冬雪,女,在读硕士生
  • 基金资助:
    *国家自然科学基金青年科学基金项目(No.82004355),中国中医科学院西苑医院苗圃课题(No.2019XYMP-15),中国中医科学院优秀青年科技人才培养专项(No.ZZ14-YQ-005)

Study on the expression levels of Ghrelin/GHSR-1a/GABA pathway key factors in models of gastroesophageal reflux disease with liver constraint pattern*

Chen Dongxue1, Qian Zhanhong1, Xia Congyuan2, Ma Ru3, Xu Yuan2, Zhang Liying4#   

  1. 1 School of Traditional Chinese Medicine, Inner Mongolia Medical University, Inner Mongolian 010110, China;
    2 Department of TCM Rheumatology & Department of Pharmacy, China-Japan Friendship Hospital, Beijing 100029, China;
    3 Beijing University of Chinese Medicine, Beijing 100029, China;
    4 Department of Spleen-stomach Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China
  • Received:2021-01-03 Online:2021-06-30 Published:2021-06-25
  • Contact: Zhang Liying, Ph.D., Attending Physician. Xiyuan Hospital, China Academy of Chinese Medical Sciences. No.1 Xiyuangcaochang Road, Haidian District, Beijing 100091. E-Mail:zhangliying2000@163.com
  • Supported by:
    National Natural Science Foundation for Young Scientists of China (No. 82004355)

摘要: 目的 研究胃饥饿素/生长激素促分泌性受体/γ-氨基丁酸(Ghrelin/GHSR-1a/GABA)通路的关键因子在胃食管反流病肝郁证大鼠模型中的表达情况,建立相关病证结合动物模型。方法 将55只雄性SD大鼠随机分为正常组、假手术组、胃食管反流病组、肝郁证组、胃食管反流病肝郁证组,每组11只。以食管十二指肠侧吻合术制备胃食管反流病模型,以慢性不可预见性刺激制备中医肝郁证模型,以食管十二指肠侧吻合术联合慢性不可预见性刺激制备胃食管反流病肝郁证模型。进行糖水偏爱实验、旷场实验、食管上皮HE病理观察,检测血清及胃组织Ghrelin、下丘脑GABA和GHSR-1a表达。结果 分别与正常组、胃食管反流病组比较,胃食管反流病肝郁证组大鼠糖水偏好比和旷场实验总穿格数降低(P<0.01)、中央区持续停留时间缩短(P<0.01),血清中Ghrelin含量增高(P<0.01),胃组织中Ghrelin阳性细胞表达数及下丘脑GHSR-1a蛋白表达增加(P<0.01)。与假手术组比较,胃食管反流病肝郁证组大鼠下丘脑中GABA含量降低(P<0.05)。结论 食管十二指肠侧吻合术联合慢性不可预见性刺激可以制备胃食管反流病肝郁证模型,该病证结合大鼠模型血清及胃Ghrelin表达增高,下丘脑GHSR-1a蛋白表达增高、GABA含量降低。

关键词: 胃食管反流病, 肝郁证, 胃饥饿素, 生长激素促分泌性受体, γ-氨基丁酸, 病证结合模型, 大鼠

Abstract: Objective To investigate the expression levels of Ghrelin/GHSR-1a/GABA pathway key factors in rat model of gastroesophageal reflux disease (GERD) with liver constraint pattern. Methods 55 SD rats were randomly divided into the normal group, sham operation group, GERD group, liver constraint pattern group, and GERD with liver constraint pattern group, with 11 rats in each group. The GERD models were established by esophagogastroduodenal anastomosis (EGDA), the liver constraint pattern models were established by chronic unpredictable stimulation (CUS), and the GERD with liver constraint pattern models were established by EGDA combined with CUS. Sucrose preference test, open field test, and HE pathological observation of esophageal epithelium were performed. Ghrelin levels in serum and gastric tissue, GABA and GHSR-1a expressions in the hypothalamus was detected. Results Compared with the normal group and the GERD group respectively, the sucrose preference percentage and the total number of grids crossed (P<0.01) and the time spent in the central zone (P<0.01) by the GERD with liver constraint pattern group in the open field test were reduced, and the content of Ghrelin in the serum (P<0.01), the expressions of Ghrelin positive cells in the gastric tissue, and the expressions of GHSR-1a protein in the hypothalamus (P<0.01) were increased. Compared with the sham operation group, the contents of GABA in the hypothalamus of rats in the GERD with liver constraint pattern group were reduced (P<0.05). Conclusion EGDA combined with CUS can successfully establish the GERD with liver constraint pattern models. The expressions of the Ghrelin in the serum and gastric tissue and the GHSR-1a protein in the hypothalamus in the rat models may increase while the contents of GABA may decrease.

Key words: gastroesophageal reflux disease, liver constraint pattern, Ghrelin, GHSR-1a, GABA, model of combination of disease and pattern, rats

中图分类号: 

  • R228