主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2021, Vol. 44 ›› Issue (9): 837-845.doi: 10.3969/j.issn.1006-2157.2021.09.012

• 中药药理 • 上一篇    下一篇

调肝理肺法通过HPA轴对心理应激哮喘大鼠Th1/Th2调节作用*

李长安1, 崔红生2#, 弓雪峰2, 辛芳3   

  1. 1 北京中医药大学东直门医院 北京 100700;
    2 北京中医药大学第三附属医院;
    3 北京市朝阳区望京社区卫生服务中心
  • 收稿日期:2021-04-09 出版日期:2021-09-30 发布日期:2021-10-08
  • 通讯作者: #崔红生,男,博士,教授,主任医师,博士生导师,主要研究方向:中医药防治肺系疾病,E-mail:hshcui@sina.com
  • 作者简介:李长安,男,博士,主治医师
  • 基金资助:
    *国家自然科学基金面上项目(No.82074390),中央高校基本科研业务费专项资金资助(No.2018-JYB-XJS-078)

Regulatory effects of regulating liver and lung by means of the HPA axis on Th1/Th2 of asthmatic rats with psychological stress*

Li Chang'an1, Cui Hongsheng2#, Gong Xuefeng2, Xin Fang3   

  1. 1 Dongzhimen Hospital Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, China;
    3 Wangjing Community Health Service Center, Beijing 100101, China
  • Received:2021-04-09 Online:2021-09-30 Published:2021-10-08
  • Contact: Prof. Cui Hongsheng,Ph.D., Doctoral Supervisor.Beijing University of Chinese Medicine Third Affiliated Hospital. No. 51 Xiaoguan Road, Chaoyang District, Beijing 100029. E-mail: hshcui@sina.com
  • Supported by:
    National Natural Science Foundation of China(No.82074390); The Fundamental Research Funds for the Central Universities (No. 2018-JYB-XJS-078)

摘要: 目的 通过哮喘宁颗粒对心理应激哮喘大鼠进行干预,观察实验动物血清、灌洗液、肺组织相关指标变化,阐明调肝理肺法通过下丘脑-垂体-肾上腺(HPA)轴对心理应激哮喘大鼠Th1/Th2调节作用。方法 将大鼠随机分为正常组、应激哮喘组、普米克令舒组和哮喘宁组,每组8只。应激哮喘组予致敏及雾化激发14 d,并同时给予束缚制动,连续28 d。哮喘宁组在心理应激哮喘大鼠模型的基础上,从实验第15天开始每天激发前2 h给予普米克令舒363.11 μg/kg雾化吸入,连续14 d。哮喘宁组在心理应激哮喘大鼠模型的基础上,从实验第15天开始每天激发前2 h给予哮喘宁颗粒2.18 g/kg灌胃,连续14 d。正常组致敏和激发均以生理盐水,但不做束缚刺激。通过流式细胞技术、ELISA法、免疫组化法、Western blotting技术等检测血清及支气管肺泡灌洗液(BALF)、肺组织中相关指标浓度、阳性细胞表达及蛋白表达的变化情况,研究调肝理肺法对心理应激哮喘大鼠HPA功能及免疫炎症的调节作用。结果 与应激哮喘组相比较,哮喘宁组静脉血嗜酸粒细胞明显降低(P<0.01);肺组织中炎症细胞浸润明显减少。哮喘宁组大鼠血清、BALF内促肾上腺皮质激素释放素(CRH)、促肾上腺皮质激素(ACTH)、皮质酮(CORT)含量较应激哮喘组明显下降(P<0.01),而肺组织糖皮质激素受体(GR)阳性表达明显增高(P<0.01),其含量轻度升高,差异无统计学差异(P>0.05)。而血清、BALF及支气管肺组织白细胞介素4(IL-4)、白细胞介素17(IL-17)含量及阳性细胞表达明显下降(P<0.01);肺组织γ干扰素(IFN-γ)阳性细胞表达明显降低(P<0.01),而含量略有降低,差异无统计学意义(P>0.05)。结论 调肝理肺法可能通过调控HPA轴功能,减轻气道炎症反应,从而实现对心理应激哮喘的治疗作用。

关键词: 调肝理肺法, 心理应激, 哮喘, 下丘脑-垂体-肾上腺轴, 大鼠

Abstract: Objective The changes in serum, lavage fluid and lung tissue were observed in model rats treated with Xiaochuan Ning (Asthma-relieving) Granule to explore the regulatory effects of regulating liver and lung by means of the HPA axis on Th1/Th2 of asthmatic rats with psychological stress.Methods SD rats were randomly divided into the normal group, asthma with psychological stress (APS) group, Pulmicort Respules (PR) group and Xiaochuan Ning Granule (XCNG) group, with 8 rats in each group. The APS group was sensitized with ovalbumin (OVA) and luminum hydroxide adjuvant and stimulated with atomized OVA for 14 days, and restrained simultaneously for 28 consecutive days.The WM group was given atomized Pulmicert Respules 363.11μG/kg 2 hours before the daily stimulation from the 15th day of the experiment for 14 consecutive days in addition to the same intervention given to the APS group. The XCNG group was treated with Xiaochuan Ning Granule 2.18 g/kg by gavage 2 hours before the daily stimulation from the 15th day of the experiment for 14 consecutive days in addition to the same intervention given to the APS group. The normal group was sensitized and stimulated with saline, but was not restrained. Changes in the concentrations of relevant indicators, positive cell expressions and protein expressions in the serum, bronchoalveolar lavage fluid (BALF) and lung tissue were detected with flow cytometry, ELISA, immunohistochemistry and Western blotting to investigate the regulatory effects of regulating liver and lung on the function of hypothalamic pituitary adrenal axis (HPA) and immune inflammation in psychologically stressed asthmatic rats. Results Compared with The APS group, the peripheral blood eosinophil counts were significantly reduced (P<0.01) and the infiltration of inflammatory cells in lung tissue was significantly reduced of the rats in the XCNG group. The levels of CRH, ACTH and CORT in the serum and BALF of the rats in the XCNG group were significantly lower than those in the APS group (P<0.01), while the GR positive expressions were significantly higher than that in the APS group (P<0.01) though the content was only slightly higher without statistical significance (P>0.05). On the other hand, the levels of IL-4 and IL-17 in serum, BALF and bronchial lung tissue were significantly decreased (P<0.01), and the expression of IFN-γ positive cells in lung tissue was significantly decreased (P<0.01) but the content was only slightly decreased without statistical significance (P>0.05).Conclusion Regulating liver and lung may be able to treat asthma with psychological stress by regulating the HPA axis.

Key words: regulating liver and lung, psychological stress, bronchial asthma, hypothalamic-pituitary-adre-nal axis, rats

中图分类号: 

  • R285.5