主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2014, Vol. 37 ›› Issue (4): 285-288.doi: 10.3969/j.issn.1006-2157.2014.01.017

• 针灸 • 上一篇    

针刺对急性心肌缺血大鼠心肌组织氯离子通道调控蛋白CFTR、ClC-2表达的影响

邵晓姣1, 侯国亮1, 梁繁荣2, 陈以国1, 成泽东1   

  1. 1 辽宁中医药大学 辽宁 110847;
    2 成都中医药大学
  • 收稿日期:2013-07-19 出版日期:2014-04-30 发布日期:2014-04-30
  • 通讯作者: 成泽东,男,博士,教授,硕士生导师,研究方向:针灸机理研究
  • 作者简介:邵晓姣,女,在读硕士生
  • 基金资助:
    国家重点基础研究发展计划(973计划)资助项目(No.2012CB518503)

Influences of acupuncture on expressions of myocardial CFTR and ClC-2 in rats with acute myocardial ischemia

SHAO Xiao-jiao1, HOU Guo-liang1, LIANG Fan-rong2, CHEN Yi-guo1, CHENG Ze-dong1   

  1. 1 Liaoning University of Traditional Chinese Medicine, Liaoning 110847;
    2 Chengdu University of Traditional Chinese Medicine
  • Received:2013-07-19 Online:2014-04-30 Published:2014-04-30

摘要: 目的 观察循经取穴针刺对急性心肌缺血大鼠心肌组织氯离子(Cl-)通道囊性纤维化跨膜转导调节因子(CFTR)、ClC-2型氯通道(ClC-2)蛋白表达的影响,探讨经穴效应循经特异性机制。方法 60只SD大鼠随机分为5组:空白组、模型组、内关组、列缺组和非经非穴组。除空白组外,其余4组大鼠均采用多点皮下注射异丙肾上腺素(ISO)方法建立急性心肌缺血模型。造模成功后,内关组、列缺组、非经非穴组大鼠分别电针双侧内关穴、列缺穴、非经非穴(取天枢穴与神阙穴的连线中点),每日1次,连续7 d。采用HE染色法物镜下观察各组大鼠心脏组织结构变化,试剂盒方法检测大鼠心肌组织肌酸激酶,同工酶(CK-MB)含量,ELISA法检测大鼠血清丙二醛(MDA)含量,Western blot免疫印迹法检测大鼠心肌组织氯离子通道CFTR、ClC-2的蛋白表达。结果 心肌组织HE染色切片观察显示:模型组、列缺组和非经非穴组心肌细胞心肌纤维断裂、排列紊乱,肌浆肿胀,明显炎症细胞浸润,有许多棕褐色颗粒广泛分布在细胞核中;与模型组比较,内关组则有明显改善。与空白组比较,模型组,列缺组,非经非穴组氯离子通道CFTR和ClC-2蛋白表达显著增多(P<0.01),CK-MB含量显著升高(P<0.01),血清MDA含量升高(P<0.05);与模型组比较,内关组大鼠心肌组织CK-MB含量降低(P<0.05),血清MDA含量降低(P<0.05),氯离子通道CFTR、ClC-2蛋白表达显著减弱(P<0.01)。结论 循经针刺内关穴能有效的减轻大鼠心肌缺血引起的病理变化,调节心肌组织氯离子通道CFTR、ClC-2的蛋白表达可能是其作用机制之一。

关键词: 针刺, 急性心肌缺血, 内关穴, 心肌组织, 囊性纤维化跨膜转导调节因子, 大鼠

Abstract: Objective To observe the influences of acupuncture on the protein expressions of myocardial CFTR and ClC-2 (regulatory proteins of chloride channel) in rats with acute myocardial ischemia (AMI), and discuss the mechanism of specificity of selecting acupoints along meridians. Methods SD rats (n=60) were randomly divided into blank group, model group, Neiguan (PC6) group, Lieque (LU7) group and non-acupoint group. Except of blank group, other groups were given multiple subcutaneous injection of isoproterenol for establishing AMI model. The Neiguan group, Lieque group and non-acupoint group were given electro-acupuncture in bilateral Neiguan, Lieque and middle point between Tianshu (ST25) and Shenque (CV8) once a day for 7 d. The changes of myocardial histology were observed by using microscope after HE staining. The content of creative kinase (CK-MB) was detected by using kit method, content of malonyldialdehyde (MDA) was detected by using EELISA, and protein expressions of CFTR and ClC-2 were detected by using Western blot method. Results The HE staining sections of myocardial tissue showed that fragmentation and disordered arrangement of myocardial fibers, sarcoplasm swelling, obvious inflammatory cell infiltration, and wide distribution of many brown granules in nucleus in model group, Lieque group and non-acupoint group, which were relieved in Neiguan group compared with model group. Compared with blank group, the protein expressions of CFTR and ClC-2 increased significantly (P<0.01), CK-MB content increased significantly (P<0.01) and MDA content increased in model group, Lieque group and non-acupoint group. Compared with model group, CK-MB content decreased (P<0.05), MDA content decreased (P<0.05) and expressions of CFTR and ClC-2 decreased significantly (P<0.01) in Neiguan group. Conclusion Acupuncture in Neiguan can effectively relieve the pathological changes induced by myocardial ischemia, and the mechanism may be related to regulating the protein expressions of CFTR and ClC-2.

Key words: acupuncture, acute myocardial ischemia, Neiguan (PC6), myocardial tissue, CFTR, rats

中图分类号: 

  • R245