主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (5): 318-322.doi: 10.3969/j.issn.1006-2157.2015.05.007

• 科技之窗 • 上一篇    下一篇

三七总皂苷对高糖诱导大鼠视网膜微血管内皮细胞损伤的保护作用*

乔园,段惠惠,黄建梅,唐民科#   

  1. 北京中医药大学中药学院 北京 100102
  • 收稿日期:2015-03-04 出版日期:2015-05-15 发布日期:2015-05-15
  • 通讯作者: 唐民科,男,教授,博士生导师,研究方向:神经药理学,E-mail: tangmk@bucm.edu.cn
  • 作者简介:乔园,女,在读硕士生
  • 基金资助:
    *“重大新药创制”国家科技重大专项(No.2011ZX09201-201-22), 北京中医药大学研究生自主课题(No. 2014-JY13ZZ-XS-099)

Protective effects of total saponrins in Panax Notoginseng on high glucose-induced injury in cultured rat retinal capillary endothelial cells(RRCECs)*

QIAO Yuan, DUAN Hui-hui, HUANG Jian-mei, TANG Min-ke#   

  1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2015-03-04 Online:2015-05-15 Published:2015-05-15

摘要: 目的 观察三七总皂苷(PNS)对高浓度葡萄糖致大鼠视网膜微血管内皮细胞(RRCECs)损伤的保护作用和对细胞黏附分子-1(ICAM-1)的影响。方法 体外原代培养RRCECs,并通过免疫荧光化学方法鉴定;用PNS作用于4~6代的RRCECs,实验分为正常组、高糖组(30 mmol/L)和高糖不同浓度PNS(20、50、100 mg/L)组,分别使用MTT比色法和台盼蓝计数法观察PNS对细胞活力及数目的影响,使用Western blotting检测药物作用前后ICAM-1的表达变化。结果 MTT比色法结果显示,PNS 50、100 mg/L作用48 h和72 h可明显减轻高糖造成的RRCECs损伤,提高RRCECs活力(P<0.01);台盼蓝计数结果显示,在PNS作用48 h后PNS 100 mg/L 组细胞数目明显增加(P<0.01),72 h后 PNS 50、100 mg/L 组细胞数目均明显增加(P<0.05)。Western blotting 检测显示,随着PNS药物浓度的增高,高糖环境下RRCECs中的ICAM-1表达量逐渐减少(P<0.05)。结论 PNS可以减轻高糖引起的RRCECs损伤,减少高糖环境下RRCECs中ICAM-1表达。PNS对RRCECs的保护作用与ICAM-1表达调节之间的关系有待进一步研究。

关键词: 三七总皂苷, 视网膜微血管内皮细胞, 细胞间黏附分子-1, 大鼠

Abstract: Objective To observe the effects of total saponins of Panax Notoginseng (PNS) protection and expression of intercellular adhesion molecules-1 (ICAM-1) in cultured rat retinal capillary endothelial cells (RRCECs) induced with high-concentration glucose. Methods Endothelial cells obtained from rat retinas were cultured in vitro, and the cells were identified using immunofluorescence. RRCECs were randomly divided into five groups: normal group, high glucose group, high glucose+20 mg/L PNS group, high glucose+50 mg/L PNS group, and high glucose+100 mg/L PNS group. Cell viability was measured with MTT method while cell number was detected with Trypan blue assay. The protein expression of ICAM-1 in RRCECs was measured by using Western blotting. Results According to MTT assay, the cell viability in high glucose+PNS (50 mg/L, 100 mg/L) groups were promoted after an action time of 48 hours and 72 hours (P<0.01). According to Trypan blue assay, after 48 hours and 72 hours, the cell number in high glucose+PNS (100 mg/L) group was increased (P<0.01); after 48 hours, the cell number in high glucose+PNS (50 mg/L) group was increased (P<0.05). After an action time of 72 hours, the expression of ICAM-1 in all high glucose+PNS groups were detected by using Western blotting. The result showed that the expression of ICAM-1 in RRCECs decreased with the rise of PNS concentration under high glucose condition (P<0.01). Conclusion PNS could protect RRCECs from damage caused by high glucose, and inhibit the expression of ICAM-1. Relation between protective effects of PNS and regulation of ICAM-1 expression in cultured RRCECs merits further study.

Key words: total Saponins of Panax Notoginseng, retinal capillary endothelial cells, intercellular adhesion molecules-1

中图分类号: 

  • R285.5