主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (6): 383-387.doi: 10.3969/j.issn.1006-2157.2015.06.005

• 科技之窗 • 上一篇    下一篇

疏肝清热健脾法对广泛性焦虑大鼠脑内bax、bcl-2表达的影响*

董宁1,唐启盛2#,赵瑞珍2,罗斌2,王雯龙1   

  1. 1 北京中医药大学 北京 100029;
    2 北京中医药大学第三附属医院
  • 收稿日期:2014-10-20 出版日期:2015-06-15 发布日期:2015-06-15
  • 通讯作者: 唐启盛,男,博士,教授,主任医师,博士生导师,研究方向:中医药防治脑病的研究,E-mail:tangqisheng@263.com
  • 作者简介:董宁,男,在读博士生
  • 基金资助:
    *国家自然科学基金资助项目(No.81173231),高等学校博士学科点专项资助项目(No.20130013130002),北京中医药大学科研创新团队资助项目(No.2011-CXTD-26),北京中医药大学自主选题资助项目(No.2014-JYB22-JS-065)

Effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of cerebral bax and bcl-2 in rats with generalized anxiety disease*

DONG Ning1, TANG Qi-sheng2#, ZHAO Rui-zhen2, LUO Bin2, WANG Wen-long1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 The Third Affiliated Hospital, Beijing University of Chinese Medicine
  • Received:2014-10-20 Online:2015-06-15 Published:2015-06-15

摘要: 目的 研究疏肝清热健脾法对广泛性焦虑(GAD)大鼠脑内Papez环路区域的B细胞白血病/淋巴瘤-2(bcl-2)、B细胞白血病/淋巴瘤-2相关蛋白(bax)2种蛋白表达的影响。方法 将40只雄性Wistar大鼠随机分为正常组、模型组、中药治疗组和西药对照组4组,每组10只大鼠。模型组、治疗组、对照组采用不确定空瓶饮水刺激方法制备广泛性焦虑大鼠模型。治疗组和对照组分别以丹栀逍遥散(10.5 g/kg)和盐酸丁螺环酮(2 mg/kg)水溶液灌胃7 d,每天1次。模型组蒸馏水灌胃7 d。取脑组织,western blot法检测大鼠脑内海马和乳头体组织bax和bcl-2的含量。结果 与正常组比较,模型组海马和乳头体组织的bax含量显著增加,差异有统计学意义(P<0.05或P<0.01),bcl-2的含量有较明显的下降,差异有统计学意义(P<0.01);与模型组相比,治疗组海马及乳头体组织的bax表达均有降低,差异有统计学意义(P<0.05或P<0.01),bcl-2的表达均有增加,差异有统计学意义(P<0.01)。结论 以丹栀逍遥散为代表的疏肝清热健脾法能够降低GAD大鼠脑内海马和乳头体组织bax的水平,升高bcl-2的水平,降低bax/bcl-2值,干预和抑制Papez环路区域的细胞凋亡,从而改善焦虑症状。

关键词: 疏肝清热健脾法, 广泛性焦虑障碍, Papez环路, B细胞白血病/淋巴瘤-2蛋白, B细胞白血病/淋巴瘤-2相关蛋白, 大鼠

Abstract: Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez’s circuit in rat with generalized Anxiety Disease (GAD). Methods A total of 40 male Wistar rats were divided into normal group, model group, treatment group, and control group, with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragastrically given Danzhi Xiaoyao San (Cortex Moutan and Fructus Gardeniae Free Wanderer Powder) and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given distilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group (P<0.05 or P<0.01) and the expression of bcl-2 decreased markedly (P<0.01). Compared with model group, the level of bax in hippocampus and mamillary body of treatment group and control group was reduced (P<0.05 or P<0.01) and the level of bcl-2 increased significantly (P<0.05 or P<0.01). Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain, elevate the level of bcl-2 and reduce bax/bcl-2, prevent and inhibit cell apoptosis in Papez’s circuit and then ameliorate anxiety symptoms.

Key words: liver-soothing heat-clearing and spleen-fortifying method, generalized anxiety disease, Papez’s circuit, bcl-2, bax, rats

中图分类号: 

  • R285.5