主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (6): 388-392.doi: 10.3969/j.issn.1006-2157.2015.06.006

• 科技之窗 • 上一篇    下一篇

截断疗法与常规疗法对小鼠流感病毒性肺炎治疗作用的比较*

董莹莹,吴珺,陈丹军,李根茂,葛东宇,邱泽计,郝钰#   

  1. 北京中医药大学基础医学院 北京 100029
  • 收稿日期:2014-12-25 出版日期:2015-06-15 发布日期:2015-06-15
  • 通讯作者: 郝钰,女,博士,教授,博士生导师,研究方向:中医药抗病毒,E-mail: yuhao64@sina.com
  • 作者简介:董莹莹,女,在读硕士生
  • 基金资助:
    *国家自然科学基金资助项目(No.30772871),教育部博士点博导类基金资助项目(No.20130013110010),北京中医药大学自主选题资助项目(No.2014-JYBZZ-XS-020).

Comparing truncated and routine methods in the treatment of mice pneumonia induced by influenza virus*

DONG Ying-ying, WU Jun, CHEN Dan-jun, LI Gen-mao, GE Dong-yu, QIU Ze-ji, HAO Yu#   

  1. School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029
  • Received:2014-12-25 Online:2015-06-15 Published:2015-06-15

摘要: 目的 比较截断疗法和常规疗法对小鼠流感病毒性肺炎的治疗作用。方法 ICR小鼠80只,分为正常组、模型组、常规疗法组、截断疗法组,以流感病毒鼠肺适应株FM1病毒液滴鼻感染后1 h灌胃给药,截断疗法组以犀角地黄汤合银翘散水煎剂灌胃;常规疗法组第1、2、3天以银翘散水煎剂灌胃,第4、5、6、7天以犀角地黄汤水煎剂灌胃,连续给药7 d,观察14 d,计算小鼠的生存率、平均存活天数、体重变化。Balb/c 小鼠128只,分为以上4组,处理同上,分别于第2、4、6、8天处死动物并取材、检测。观察不同时间点小鼠肺组织病毒滴度、肺指数;观察第8天小鼠肺大体及肺组织病理变化。结果 截断疗法组与常规疗法组比较,生存率升高一倍,平均存活天数明显升高;截断疗法组在第6、8天较常规疗法组肺指数明显降低,在第2、4、6、8天肺组织病毒滴度较常规疗法组均有所下降,但无统计学意义。结合肉眼观察肺大体病变及光镜下观察小鼠肺部病理改变,截断疗法组病变较常规疗法组显著减轻。结论 截断疗法对重症小鼠流感病毒性肺炎的治疗作用优于常规疗法,其机制可能不在于对病毒的抑制作用强,而在于对病毒感染后机体发生的炎症级联反应抑制作用强。

关键词: 截断疗法, 病毒性肺炎, 犀角地黄汤合银翘散, 流感病毒, 小鼠

Abstract: Objective To compare the effects of truncated and routine methods in the treatment of mice pneumonia induced by influenza virus. Methods ICR mice (n=80) were randomly divided into normal group, model group, routine treatment group and truncated treatment group. The last three groups were infected with mouse lung-adapted influenza virus strain by inoculating intranasally. One hour after the inoculation, the truncated treatment group was intragastrically given Xijiao dihuang Tang (Rhinoceros Horn and Rehmannia Decoction) combined with Yinqiao San (Lonicera and Forsythia Powder) decoction while the routine treatment group was intragastrically given Yinqiao San decoction only on the first three days and Xijiao dihuang Tang only on the following four days. All groups were treated for 7 consecutive days. The survival rate, average survival days and body weights were calculated during 14 days’ observation. Balb/c mice (n=128) were divided into the same four groups described above with the same corresponding intervention. These mice were sacrificed on the 2nd, 4th, 6th and 8th days respectively. Specimen were obtained and evaluated. The viral titers in lung homogenate and the lung index were measured dynamically; the lung gross lesions and tissue pathological changes were observed on the 8th day. Results The survival rate of truncated treatment group was twice as high as routine treatment group and average survival days also increased significantly; the lung index of truncated therapy group significantly decreased compared with the routine group on the 8th day. The viral titers of truncated treatment group in lung homogenate decreased on the 2nd, 4th, 6th and 8th day compared with the routine treatment group, yet the difference was not statistically significant. The pulmonary gross lesions and tissue pathological changes under light microscope were less severe than the routine treatment group. Conclusion Truncated method seemed to be superior to routine method in the treatment of severe influenza viral pneumonia mice. Its mechanisms may not lie in its stronger anti-viral effect, but in its stronger inhibitory effect on inflammatory cascade after viral infection compared with routine treatment.

Key words: truncated method, viral pneumonia, Xijiao dihuang Tang combined with Yinqiao San, influenza virus, mice

中图分类号: 

  • R285.5