主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (7): 481-485.doi: 10.3969/j.issn.1006-2157.2015.07.011

• 针灸 • 上一篇    下一篇

不同时机介入电针对模拟失重大鼠肝脏组织结构、功能和一氧化氮水平的影响

宋艳1, 赵国桢2, 汪德生3, 赵百孝1, 嵇波1#, 张鹤1, 毛颖秋1, 张平1许咏思1, 刘亚莉1, 路雅雯1, 戴健1, , 李莹辉3   

  1. 1 北京中医药大学 北京 100029;
    2 首都医科大学;
    3 中国航天员科研训练中心
  • 收稿日期:2014-12-31 出版日期:2015-07-30 发布日期:2015-07-30
  • 通讯作者: 嵇波, 女, 博士, 教授, 博士生导师, 研究方向:经穴脏腑相关临床和基础研究, E-mail:jibo@163bj.com
  • 作者简介:宋艳, 女, 在读硕士生
  • 基金资助:
    *航天医学基础与应用国家重点实验室研究基金资助项目(No.SMFA 11B01)

Effects of electroacupuncture intervention at different time-points on the histological structure, function and level of nitric oxide of the liver in simulated weightlessness rats

SONG Yan1, ZHAO Guo-zhen2, WANG De-sheng3, ZHAO Bai-xiao1, JI Bo1#, ZHANG He1, MAO Ying-qiu1, ZHANG Ping1, XU Yong-si1, LIU Ya-li1, LU Ya-wen1, DAI Jian1, LI Ying-hui3   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 Capital Medical University;
    3 China Astronaut Research and Training Center
  • Received:2014-12-31 Online:2015-07-30 Published:2015-07-30

摘要: 目的 观察预针刺和针刺治疗对模拟失重大鼠肝脏组织结构、功能和一氧化氮(NO)水平的影响, 探讨不同时机介入电针治疗对模拟失重大鼠肝脏损伤的防护机制。方法 将20只雄性清洁级Wistar大鼠随机分为正常组、模型组、预针刺组和针刺组, 每组5只。正常组不做任何处理, 自由活动饲养4周;模型组、预针刺组和针刺组大鼠尾部悬吊4周制造模拟失重大鼠模型, 预针刺组在尾吊前1周, 电针刺激双侧“肾俞”“脾俞”和“三阴交”穴, 每次30 min, 每日1次, 共治疗7次;针刺组针刺治疗与尾部悬吊同时开始, 大鼠尾部悬吊过程中, 电针刺激“肾俞”“脾俞”和“三阴交”穴, 每次30 min, 隔日治疗1次, 共针刺14次。采用HE染色法物镜下观察各组大鼠肝脏组织结构的变化, 比色法检测各组大鼠血清谷丙转氨酶(ALT)活性及肝脏组织一氧化氮(NO)含量。结果 肝脏组织HE染色切片观察显示:模型组大鼠肝脏中央静脉周围出现大面积的肝细胞变性;经电针治疗后, 大鼠肝脏组织结构病理变化较模型组有所改善, 且预针刺组疗效优于针刺组。与正常组比较, 模型组大鼠血清ALT活性和肝脏组织NO含量显著升高(P<0.05, P<0.01);与模型组比较, 预针刺组大鼠血清ALT活性及肝脏组织NO含量显著降低(均P<0.05), 针刺组以上指标变化不显著。与预针刺组比较, 针刺组ALT活性有升高趋势, NO含量显著升高(P<0.01)。结论 预针刺较针刺治疗更能有效地缓解大鼠尾部悬吊引起的肝脏组织结构和功能的损伤, 其机理可能与NO有关。

关键词: 针刺, 模拟失重, 肝脏损伤, 血清谷丙转氨酶, 一氧化氮, 大鼠

Abstract: Objective To observe the effect of needling on the structure, function and level of nitric oxide (NO) of the liver in rats with simulated weightlessness, so as to explore the protective mechanism of acupuncture on liver injury.Methods Twenty male clean grade Wistar rats were randomly divided into normal group, model group, pretreatment group and treatment group(each n=5).The rats of normal group received no treatment, and those of other three groups were tail-suspended for 4 weeks to establish weightlessness model.Electroacupuncture at the points of Shenshu (BL23), Pishu (BL20) and Sanyinjiao (SP60) once daily for 30 min was applied one week ahead of tail-suspension in pre-treatment group, while in treatment group the needling treatment applied at the same three points once every other day for 14 d was carried out at the day when the rats was tail-suspended. The changes of hepatic histological structure were observed, and the activity of alanine aminotransferase(ALT) in serum and the content of NO in liver were measured.Results The hepatic cell degeneration were observed around the central vein in model group; the degree of degeneration relieved in both electroacupuncture treatment groups. Furthermore, the curative effect of the pre-treatment group was better than the treatment group. Compared with normal group, the activity of ALT in serum and the content of NO in liver in model group increased significantly (P<0.05, P<0.01). Compared with model group, the activity of ALT in serum and the content of NO in liver in the pre-treatment group significantly reduced(P<0.05), however, those in treatment group had no obvious difference. Compared with pre-treatment group, the activity of ALT tended to increase and the content of NO increased significantly(P<0.01) in the acupuncture group. Conclusion The effects of relieving hepatic injury and dysfunction induced by weightlessness, which potentially was related to the content of NO, in the pre-treatment group were superior to those in the treatment group.

Key words: acupuncture, simulated weightlessness, liver injury, ALT, NO, rats

中图分类号: 

  • R245.9+7