主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (8): 535-538.doi: 10.3969/j.issn.1006-2157.2015.08.007

• 科技之窗 • 上一篇    下一篇

山药芡实药对不同剂量对脾虚模型小鼠免疫功能的影响*

李云1, 李伟2, 张雪廷2, 孙宇2, 张奇文1, 曾郁敏3#   

  1. 1 北京中医药大学针灸推拿学院 北京 100029;
    2 北京中医药大学附属第二临床医学院;
    3 北京中医药大学基础医学院
  • 收稿日期:2014-08-04 出版日期:2015-08-30 发布日期:2015-08-30
  • 通讯作者: 曾郁敏,女,副教授,E-mail:13717670731@163.com
  • 作者简介:李云,女,在读本科生
  • 基金资助:
    2013年国家级大学生创新创业训练计划项目 (No.201310026022)

Effects of the herbal pair Shanyao and Qianshi at different dosage ratios on spleen-deficiency mice’s immune function*

LI Yun1, LI Wei2, ZHANG Xue-ting2, SUN Yu2, ZHANG Qi-wen1, ZENG Yu-min3#   

  1. 1 School of Acupuncture, Moxibustion and Tuina, Beijing University of Chinese Medicine. Beijing 100029;
    2 The Second School of Clinical Medicine, Beijing University of Chinese Medicine;
    3 School of Preclinical Medicine, Beijing University of Chinese Medicine
  • Received:2014-08-04 Online:2015-08-30 Published:2015-08-30

摘要: 目的 比较山药芡实药对不同剂量对脾虚模型小鼠体重及免疫指标胸腺指数、T细胞增殖能力、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)的影响,探究山药芡实药对在提高免疫力方面的最佳剂量与配比。方法 将小鼠随机分为山药芡实3∶1组、山药芡实2∶1组、山药芡实1∶1组、山药芡实1∶2组4个实验组和模型组、正常组共6组,以番泻叶水煎剂灌胃对实验组和模型组进行脾虚模型的建造,实验组以不同剂量的山药芡实药对汤剂灌胃,模型组和正常组给予等量生理盐水灌胃,称重计量体重和胸腺指数,MTT比色法检测T细胞增殖能力,双抗夹心酶联免疫吸附剂测定(ELISA)法检测血清IL-2、IL-4和IFN-γ水平。结果 造模后小鼠体重明显低于正常组(P<0.05),造模成功。给药后各实验组相关免疫指标均有不同程度改善,其中山药芡实3∶1组和山药芡实2∶1组改善程度最大,尤其是血清IL-2、IL-4水平和胸腺指数(P<0.05,P<0.01),而在体重、T细胞增殖能力方面,山药芡实2∶1组改善程度大于山药芡实3∶1组。结论 山药芡实药对能通过影响免疫功能来改善小鼠脾虚状态,具有健脾益气的功效;推测山药芡实药对的临床最佳剂量与配比为2∶1(30 g∶15 g)。

关键词: 山药, 芡实, 药对, 脾虚, 免疫功能, 小鼠

Abstract: Objective To compare the effects of herbal pair, Shanyao (Rhizoma Dioscoreae) and Qianshi (Semen Euryales), at different dosage ratios on spleen-deficiency mice’s weight and immunity parameters including thymus weight, proliferation of T cells as well as IL-2, IL-4 and IFN-γ, and to study the optimal dose and best ratio of this pair in terms of immunity improvement. Methods ICR mice were randomly divided into 4 experimental groups (ratios of 3∶1, 2∶1, 1∶1 and 1∶2 respectively), model group and normal group. To establish spleen-deficiency model, the experimental groups and the model group were given senna decoction by gavage, and the normal group was given equal volume of saline by gavage. Experimental groups were then given different dosages of Qianshi and Shanyao decoction by gavage, while the model group and the normal group were given equal volume of saline by gavage. Body Mass Index and thymus index were measured by weighing, T cell proliferation ability was tested by using MTT, and the serum levels of IL-2,IL-4 and IFN-γwere tested with a double-antibody sandwich ELISA method. Results After the establishment of spleen-deficiency model, mice’s weights were significantly below normal range (P<0.05), which demonstrated success of the model establishment. After the gavages of the herbal pair, associated immune indexes in all experimental groups improved at various degrees, with the most significant improvement in 3∶1 group and 2∶1 group (P<0.05), especially their serum levels of IL-2 and IL-4 as well as the thymus index. A greater degree of improvement was observed in 2∶1 group than 3∶1 group in terms of weight and T cell proliferation ability. Conclusion The herbal pair of Qianshi and Shanyao could relieve the mice’s spleen deficiency by enhancing immune function, as it could invigorate spleen and replenish Qi. The optimal dose and best ratio of Qianshi and Shanyao were expected to be 2∶1(30 g∶15 g).

Key words: Rhizoma Dioscoreae, Semen Euryales, herbal pair, spleen-deficiency, immune function, mice

中图分类号: 

  • R285.5