主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2015, Vol. 38 ›› Issue (10): 709-715.doi: 10.3969/j.issn.1006-2157.2015.10.014

• 临床研究 • 上一篇    下一篇

Springbio蛋白芯片技术研究气虚质及鼻咽癌气虚癌变差异蛋白表达

周小军1,张丽娟1,陈江华2,汪芸3   

  1. 1.广州中医药大学附属中山医院耳鼻咽喉科 广东 528400;
    2.广州中医药大学附属中山医院检验科;
    3.广州中医药大学附属中山医院病理科
  • 收稿日期:2015-04-12 出版日期:2015-10-30 发布日期:2015-10-30
  • 作者简介:周小军,男,博士,教授,博士生导师
  • 基金资助:
    *国家自然科学基金项目(No.81072847,81373698)

Protein expression characteristics in qi-deficiency constitution and qi-deficiency pattern of nasopharyngeal cancer using springbio protein chip technology*

ZHOU Xiaojun 1, ZHANG Lijuan1, CHEN Jianghua2, WANG Yun3   

  1. 1.ENT Department, Zhongshan Hospital, Guangzhou University of Traditional Chinese Medicine, Guangdong 528400;
    2.Laboratory, Zhongshan hospital, Guangzhou University of Traditional Chinese Medicine;
    3.Pathology Department, Zhongshan Hospital, Guangzhou University of Traditional Chinese Medicine
  • Received:2015-04-12 Online:2015-10-30 Published:2015-10-30

摘要: 目的 利用蛋白芯片技术研究气虚质及鼻咽癌气虚癌变差异蛋白的表达。方法 选择具有典型气虚质的鼻咽癌及鼻咽炎各4例作为研究对象,采用可同时检测722个蛋白的Springbio蛋白芯片技术及协方差分析方法,检测气虚质差异蛋白、鼻咽癌差异蛋白以及鼻咽癌气虚癌变差异蛋白的表达情况,探索其特征蛋白。结果 相比于正常质,气虚质蛋白主要以下调表达为主,下调蛋白19个(P<0.05),气虚质下调的蛋白主要包括角蛋白及细胞周期蛋白依赖激酶;相比于鼻咽炎,鼻咽癌蛋白既可上调表达亦可下调表达,上调表达有13个蛋白,下调表达有9个蛋白;CD115/CSF-1R和角蛋白既在气虚质下调,又在鼻咽癌下调,Cdk1/p34cdc2和p170 / MDR-1蛋白在气虚质下调,在鼻咽癌却上调。结论 气虚质存在着特定的蛋白表达特征,表现为角蛋白及细胞周期蛋白依赖激酶的低表达,集落刺激因子1受体及角蛋白低表达为鼻咽癌气虚癌变特征蛋白。

关键词: 鼻咽癌, 气虚, 角蛋白, 集落刺激因子1受体, 细胞周期蛋白依赖激酶

Abstract: Objective To investigate protein expression characteristics of qi-deficiency constitution and qi deficiency-related nasopharyngeal cancer (NPC) by using protein chip technology. Methods Four cases with nasopharyngeal carcinoma and 4 cases with nasopharyngitis, all of the typical qi-deficiency constitution were examined. Springbio protein chip technology with the capacity of detecting 722 proteins was used to detect protein expressions in qi deficiency constitution, sopharyngeal carcinoma and sopharyngeal carcinoma of qi-deficiency type, so as to explore the characteristics of the protein. Results As compared with the healthy subjects, most protein expressions were down-regulated in qi deficiency patients. There had been 19 down-regulated proteins (p< 0.05), mainly including the keratin protein and cyclin dependent kinase. As compared with nasopharyngitis, 13 proteins were up-regulated while 9 were down-regulated in nasopharyngeal carcinoma protein expressions. CD115/c-fms/CSF-1R/M-CSFR and keratin showed lower expression in nasopharyngeal carcinoma and qi deficiency type. However, Cdk1 / p34cdc2 and p170 / MDR 1 protein were down-regulated in qi deficiency type, but up-regulated in nasopharyngeal carcinoma. Conclusion There is a specific protein expression characteristic in the deficiency constitution, manifesting as lowered expression of keratin and cyclin dependent kinase. Colony stimulating factor 1 receptor and keratin protein low expression are characteristics of qi deficiency related to nasopharyngeal cancer.

Key words: nasopharyngeal carcinoma, qi deficiency, keratin, colony stimulating factor 1 receptor, cyclin dependent kinase

中图分类号: 

  • R273.963