主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2016, Vol. 39 ›› Issue (7): 586-589.doi: 10.3969/j.issn.1006-2157.2016.07.012

• 中药化学 • 上一篇    下一篇

小叶金露梅对醛糖还原酶和α-葡萄糖苷酶的抑制作用*

孙玉侠1,曾阳1,张倩茹1,余涛1,李美华1,赵得萍2#   

  1. 1 青海师范大学生命与地理科学学院 青藏高原环境与资源教育部重点实验室 青海 810008;
    2 中国科学院西北高原生物研究所
  • 收稿日期:2015-12-17 出版日期:2016-07-30 发布日期:2016-07-30
  • 通讯作者: 赵得萍,女,硕士,研究方向:科技管理,E-mail: dpzhao@nwipb.cas.cn
  • 作者简介:孙玉侠,女,在读硕士生
  • 基金资助:
    *青海省自然科学基金项目(No.2015-ZJ-907);青藏高原药用动植物资源重点实验室项目(No.2014-z-y25)

Inhibition of Potentilla parvifolia on alpha-glucosidase and aldose reductase*

SUN Yuxia1, ZENG Yang1, ZHANG Qianru1, YU Tao1, LI Meihua1,ZHAO Deping2#   

  1. 1 Lab of Tibet Plateau Environment and Resources, Key Lab of Ministry of Education, School of Life Science and Geography, Qinghai Normal University, Qinghai 810008;
    2 Northwest Plateau Institute of Biology, Chinese Academy of Sciences
  • Received:2015-12-17 Online:2016-07-30 Published:2016-07-30

摘要: 目的 对小叶金露梅不同提取部位的α-葡萄糖苷酶(AG)和醛糖还原酶(AR)抑制活性进行研究,找寻天然的AG和AR抑制剂。方法 体外实验通过α-葡萄糖苷酶抑制剂和醛糖还原酶抑制剂筛选模型,对小叶金露梅乙酸乙酯部位(总黄酮)、石油醚部位和水层部位提取物的AG和AR抑制活性进行测定;体内实验进行小叶金露梅总黄酮对小鼠糖耐量的测定。结果 小叶金露梅总黄酮部位对AG和AR有较高的抑制活性,而且总黄酮部位抑制AG活性的作用强于阿卡波糖。同时,小叶金露梅总黄酮高、低剂量均能提高小鼠的糖耐量。结论 小叶金露梅总黄酮对α-葡萄糖苷酶和醛糖还原酶具有抑制活性。

关键词: 小叶金露梅, α-葡萄糖苷酶, 醛糖还原酶, 抑制活性, 糖耐量

Abstract: Objectiv To study the inhibitory effects on α-glucosidase (AG) and aldose reductase (AR) extracted from Potentilla parvifolia in different solvents, and to find out natural inhibitors of AG and AR.Methods With a combined vitro and vivo experiment design, this study measured the in vitro inhibitory effects of AG and AR extraction from Potentilla Parvifolia in three solvents: ethyl acetate (total flavonoids), petroleum ether and water through AG inhibitors and AR inhibitory activity screening models. In vivo effects of total flavonoids from Potentilla Parvifolia on impaired glucose tolerance of mice were also measured.Results Extraction of Potentilla Parvifolia in total flavonoids demonstrated a stronger inhibitory effect on AR and AG. Its inhibitory effect on AG was higher than that of acarbose. Meanwhile, both high and low dose of total flavonoids improved glucose tolerance in mice. Conclusion Total flavonoids in Potentilla parvifolia exhibits inhibitory effects on AG and AR

Key words: Potentilla parvifolia, α-glucosidase, aldose reductase, inhibitory activity, glucose tolerance

中图分类号: 

  • R284.2