主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (1): 65-70.doi: 10.3969/j.issn.1006-2157.2018.01.011

• 科技之窗 • 上一篇    下一篇

二甲双胍联合膈下逐瘀汤对多囊卵巢综合征大鼠炎性因子及性激素水平的影响*

姜晓琳1, 张兵2, 侯海燕3, 滕飞4, 马贤德4, 吕美君1, 王莹1, 褚春莉5, 董晓茜5, 张立德1#   

  1. 1 辽宁中医药大学中医藏象理论及应用教育部重点实验室 辽宁 110847;
    2 辽宁省中医院;
    3 中国人民武装警察部队后勤学院;
    4 辽宁中医药大学教学实验中心;
    5 辽宁省中医研究院;
  • 收稿日期:2017-02-19 出版日期:2018-01-30 发布日期:2018-01-30
  • 通讯作者: 张立德,男,教授,博士,博士生导师,研究方向:中医藏象理论,E-mail:zhldtcm@163.com
  • 作者简介:姜晓琳,女,在读博士生,主治医师
  • 基金资助:
    *国家自然科学基金项目(No. 81402691);辽宁省教育厅创新团队项目(No. LT2014021);中医藏象理论及应用国家教育部重点实验室开放基金(No. 11020163150009)

Influence of metformin combined with Gexia Zhuyu Tang on inflammatory factors and sex hormone level in rats with polycystic ovarian syndrome*

Jiang Xiaolin1, Zhang Bing2, Hou Haiyan3, Teng Fei4, Ma Xiande4, Lyu Meijun1, Wang Ying1, Chu Chunli5, Dong Xiaoqian5, Zhang Lide1#   

  1. 1 Key Laboratory of Ministry of Education for TCM Visceral manifestation Theory and Applications, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Hospital of Chinese Medicine of Liaoning Province, Liaoning 110032, China;
    3 Logistics College of Chinese People’s Armed Police, Tianjin 300162, China;
    4 Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    5 Liaoning Institute of Traditional Chinese Medicine, Liaoning 110000, China;
  • Received:2017-02-19 Online:2018-01-30 Published:2018-01-30
  • Supported by:
    National Natural Science Foundation of China (No. 81402691), Innovation Team of Liaoning Provincial Education Department (No. LT2014021), Key Laboratory of Ministry of Education for TCM Visceral manifestation Theory and Applications (No. 11020163150009)

摘要: 目的 研究二甲双胍联合膈下逐瘀汤对经脱氢表雄酮诱导的多囊卵巢综合征(PCOS)模型大鼠外周血清炎性因子、性激素水平及糖脂代谢的影响。方法 将69只PCOS(经脱氢表雄酮诱导)大鼠随机分为对照组(34只)和干预组(35只)。对照组大鼠每天给予二甲双胍(270 mg/kg)灌胃,干预组大鼠每天给予二甲双胍(270 mg/kg)+膈下逐瘀汤(34.5 mg/kg)灌胃,连续治疗28 d。干预结束后,用ELISA法测定2组大鼠外周血清炎性因子[白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、C反应蛋白(CRP)]、血脂[甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、总胆固醇(TC)]、血糖[空腹血糖(FPG)、餐后2 h血糖(2 hPG)]、激素水平[卵泡雌激素(FSH)、黄体生成素(LH)、雌激素(E2)、睾酮(T)];用免疫组化法测定卵巢组织中IL-6、TNF-α蛋白表达。结果 干预后2组大鼠外周血清IL-6、TNF-α、CRP、LH、LH/FSH、E2、T、TG、LDL-C、TC、FPG、2 hPG均较治疗前有所降低(P<0.05),且干预组低于对照组(P<0.05);治疗后2组大鼠外周血清HDL-C、FSH较治疗前有所升高(P<0.05),且干预组高于对照组(P<0.05);干预后干预组大鼠的IL-6、TNF-α蛋白表达低于对照组(P<0.05)。结论 二甲双胍联合膈下逐瘀汤能降低PCOS大鼠外周血清炎性因子浓度,并能改善循环中血脂、血糖水平及其激素水平,且效果优于单纯使用二甲双胍。

关键词: 二甲双胍, 膈下逐瘀汤, 多囊卵巢综合征, 炎性因子, 激素水平, 糖脂代谢, 大鼠

Abstract: Objective To study the influence of metformin combined with Gexia Zhuyu Tang (Decoction for Dissipating Blood Stasis under Diaphragm) on serum inflammatory factors, sex hormone level and glycolipid metabolism in rat model of polycystic ovarian syndrome (PCOS) induced by dehydroepiandrosterone. Methods PCOS rats (n=69) were randomly divided into control group (n=34) and intervention group (n=35). The control group was intragastrically given metformin (270 mg/kg) and intervention group, metformin (270 mg/kg) and Gexia Zhuyu Tang (34.5 mg/kg) for continuous 28 d. The changes of serum inflammatory factors [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP)], blood fat [triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C) and total cholesterol (TC)], blood sugar [fasting plasma glucose (FPG) and 2-h postprandial blood glucose (2-hPBG)], and sex hormone level [follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2) and testosterone (T) were detected by using enzyme-linked immunosorbent assay (ELISA). The protein expressions of IL-6 and TNF-α were detected by using immunohistochemistry technique. Results After intervention, the levels of IL-6, TNF-α, CRP, LH, LH/FSH, E2, T, TG, LDL-C, TC, FPG and 2 hPG all decreased in 2 groups (P<0.05), and they were all lower in intervention group than those in control group (P<0.05). The levels of HDL-C and FSH increased in 2 groups after intervention (P<0.05), and they were higher in intervention group than those in control group (P<0.05). The protein expressions of IL-6 and TNF-α were lower in intervention group than those in control group (P<0.05). Conclusion Metformin combined with Gexia Zhuyu Tang can reduce the levels of peripheral serum inflammatory factors and meliorate levels of blood fat, blood sugar and hormones in PCOS rats, and the effect of which is better than that ot sinle metformin.

Key words: metformin, Gexia Zhuyu Tang (Decoction for Dissipating Blood Stasis under Diaphragm), polycystic ovarian syndrome, inflammatory factors, hormone level, glycolipid metabolism, rats

中图分类号: 

  • R285.5