主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (7): 547-552.doi: 10.3969/j.issn.1006-2157.2018.07.004

• 博士之光 • 上一篇    下一篇

加味芍药甘草汤调控P53-273H对子宫腺肌细胞增殖的影响*

姜心禅1, 李坤寅2#, 关永格3, 王帅3, 郭宇丹4   

  1. 1 广东药科大学 广东 510006;
    2 广州中医药大学附属第三医院;
    3 广州中医药大学附属第一医院;
    4 广州中医药大学
  • 收稿日期:2017-12-27 出版日期:2018-07-30 发布日期:2018-07-30
  • 通讯作者: 李坤寅,男,博士,教授,博士生导师,E-mail: lky0303@gzucm.edu.cn
  • 作者简介:姜心禅,女,博士,讲师
  • 基金资助:
    国家自然科学基金资助项目(No. 81473715)

Influence of Jiawei Shaoyao Gancao Tang on proliferation of adenomyosis cells through regulating P53-273H*

Jiang Xinchan1, Li Kunyin2#, Guan Yongge3, Wang Shuai3, Guo Yudan4   

  1. 1 Guangdong Pharmaceutical University, Guangdong 510006, China;
    2 Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong 510360, China;
    3 First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong 510000, China;
    4 Guangzhou University of Chinese Medicine, Guangdong 510000, China
  • Received:2017-12-27 Online:2018-07-30 Published:2018-07-30
  • Supported by:
    General Program National Natural Science Foundation of China(No.81473715)

摘要: 目的 研究加味芍药甘草汤通过调控肿瘤抑制因子的突变型P53-273H对子宫腺肌细胞增殖的影响。方法 取人子宫腺肌细胞进行细胞培养,随机分为加味芍药甘草汤组、米非司酮组和空白组3组,分别给予对应的药物处理24 h和48 h后,通过流式细胞术(FCM)检测加味芍药甘草汤对人子宫腺肌病病灶细胞凋亡的影响;反转录实时定量PCR(RT-qPCR)技术检测子宫腺肌细胞P53-273H mRNA、白血病抑制因子受体(LIFR)mRNA表达的影响;蛋白质印迹法(Western blot)检测药物对细胞中P53-273H、白血病抑制因子(LIF)、信号转导与转录激活子3(STAT3)、磷酸化的信号转导与转录激活子3(p-STAT3)蛋白表达的影响。结果 FCM检测显示,加味芍药甘草汤组细胞的凋亡率明显高于空白组(P<0.01),其差异具有统计学意义。RT-qPCR检测显示,加味芍药甘草汤组细胞的P53-273H mRNA、LIFR mRNA的相对表达量较空白组低,与空白组比较P<0.05,其差异有统计学意义。Western blot检测显示,加味芍药甘草汤处理24 h和48 h后,子宫腺肌细胞中的P53-273H、LIF、STAT3、p-STAT3蛋白的表达量均低于米非司酮组和空白组,与米非司酮组比较P<0.05,与空白组比较P<0.05,差异均具有统计学意义。结论 加味芍药甘草汤能在一定程度上抑制子宫腺肌细胞的增殖、并促进其凋亡,其作用机制可能是加味芍药甘草汤通过调控P53/LIF/STAT3信号通路而实现的。

关键词: 子宫腺肌病, 加味芍药甘草汤, 细胞凋亡

Abstract: Objective To study the influence of Jiawei Shaoyao Gancao Tang (Modified Debark Peony Root and Liquorice Root Decoction, JSGT) on proliferation of adenomyosis(AM) cells through regulating P53-273H. Methods The human AM cells were collected and cultured, and then divided randomly into JSGT group, mifepristone group and blank group. After treatment with corresponding medicinals for 24 h and 48 h, the influence of JSGT on apoptosis of AM focus cells was detected by using flow cytometry (FCM). The expressions of P53-273H mRNA and LIFR mRNA of AM cells were detected by using real-time reverse transcription quantitative polymerase chain reaction (RT-PCR). The protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were detected by using Western blotting assay. Results The results of FCM showed that the apoptosis rate was significantly higher in JSGT group than that in blank group (P<0.01). The results of real-time RT-PCR showed that the relative expressions of P53-273H mRNA and LIFR mRNA were lower in JSGT group than those in blank group (P<0.05). The results of Western blotting assay showed that, after intervention with JSGT for 24 h and 48 h, the protein expressions of P53-273H, LIF, STAT3 and p-STAT3 were lower in JSGT group than those in mifepristone group (P<0.05) and blank group (P<0.05). Conclusion JSGT can inhibit the proliferation and improve the apoptosis of AM cells, and the mechanism may be related to that JSGT can regulate P53/LIF/STAT3 signal pathway.

Key words: adenomyosis, Jiawei Shaoyao Gancao Tang (Modified Debark Peony Root and Liquorice Root Decoction), apoptosis

中图分类号: 

  • R285.5