主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2018, Vol. 41 ›› Issue (10): 835-841.doi: 10.3969/j.issn.1006-2157.2018.10.008

• 中药化学 • 上一篇    下一篇

蒙药复方协日嘎-4体内外化学成分分析*

白凤, 董玉#, 李斌鑫, 赵海龙, 张天明   

  1. 内蒙古医科大学药学院 内蒙古 010110
  • 收稿日期:2018-01-12 出版日期:2018-10-30 发布日期:2018-10-30
  • 通讯作者: 董玉,女,博士,教授,博士生导师,研究方向:中药复方有效物质基础研究及药物创新,E-mail:dongyu010@ 126.com
  • 作者简介:白凤,女,在读硕士生
  • 基金资助:
    国家自然科学基金资助项目(No.81660703),内蒙古自治区科技计划重点资助项目,内蒙古自治区草原英才创新团队资助项目

Analyzing chemical composition of Mongolian compound Xieriga-4 in vivo and in vitro*

Bai Feng, Dong Yu#, Li Binxin, Zhao Hailong, Zhang Tianming   

  1. Inner Mongolia Medical University, Inner Mongolia 010110,China
  • Received:2018-01-12 Online:2018-10-30 Published:2018-10-30

摘要: 目的 建立蒙药复方协日嘎-4 体内外HPLC特征图谱,归属并鉴定其体内外化学成分。方法 取SD大鼠,灌胃一定量的蒙药复方协日嘎-4制备体内含药血清和代谢尿液,通过HPLC 法进行测定,比对含药血清和尿液中有效组分,建立蒙药复方协日嘎-4 体内外HPLC 特征图谱。结果 分析蒙药复方协日嘎-4体外HPLC特征图谱,共发现了53个峰;蒙药复方协日嘎-4体内给药后出现了35个入血成分,25个为体外成分直接入血,推测另外10个为新生代谢产物。7、9、12、14、15、17、26、27、30、33、51、53号峰来源于姜黄,18、19号峰来源于黄柏,1、2、3、10、16 号峰来源于栀子,8、18、31、32 号峰来源于蒺藜。通过体内外化学成分比对鉴定出4个入血化学成分,其中3个与代谢中成分一致,推测其可能以原型成分发挥作用并排泄。结论 入血成分及代谢产物可能是复方发挥药效的有效成分,为进一步探究蒙药复方协日嘎-4治疗疾病的机制提供依据。

关键词: 蒙药复方协日嘎-4, 特征图谱, 入血成分, 代谢成分, 体内外化学成分, 大鼠

Abstract: Objective To establish the in vitro and in vivo HPLC profiles of Mongolian drug compound Xieriga-4, and assign and identify its chemical composition in vitro and in vivo. Methods To take SD rats and intragastrically administer a certain amount of Mongolian medicine compound Xieriga-4 to prepare in vivo serum and metabolic urine, and measure with HPLC. comparing the effective components in drug-containing serum and urine, an in vitro and in vivo HPLC profile of Mongolian drug compound Xieriga-4 was established. Results The in vitro HPLC profiles of Mongolian medicine compound Xieriga-4 were analyzed, and a total of 53 peaks were found. There were 35 blood components after in vivo administration of Mongolian Medicine Compound Xieriga-4, and 25 blood components were directly injected into the blood. The other 10 were considered new metabolites. Peaks 7, 9, 12, 14, 15, 17, 26, 27, 30, 33, 51, 53 originated from turmeric, Peaks 18 and 19 came from Cork; the peaks 1, 2, 3, 10, and 16 from lice; the peaks 8, 18, 31, and 32 from earthworms. Four incoming chemical constituents were identified through in vitro and in vivo chemical composition comparisons. Three of them were identical to those in metabolism and it was assumed that they may act as prototype components and be excreted. Conclusion Blood components and metabolites may be effective components of compound effects. This article speculates that the composition of blood components of the compound into the blood to speculate the mechanism of its treatment, in order to provide further evidence to explore the mechanism of Mongolian medicine compound Xieriga-4 treatment of diseases.

Key words: Mongolian Medicine Compound Xieriga-4, characteristic map, blood components, metabolic components, chemical composition in vitro and in vivo, rats

中图分类号: 

  • R284.1