主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

北京中医药大学学报 ›› 2020, Vol. 43 ›› Issue (12): 1011-1017.doi: 10.3969/j.issn.1006-2157.2020.12.007

• 中药药理 • 上一篇    下一篇

通络止痛凝胶对不同程度膝关节骨性关节炎家兔滑膜炎性程度的影响*

陈谱, 阮安民, 周俊, 张晓哲, 彭浩轩, 杨统杰, 杨艺, 蔡高凯, 王庆甫#   

  1. 北京中医药大学第三附属医院 北京 100029
  • 收稿日期:2020-06-11 出版日期:2020-12-30 发布日期:2021-01-05
  • 通讯作者: #王庆甫,男,硕士,主任医师,教授,博士生导师,主要研究方向:中医药防治骨关节退变,E-mail:qingpu-wang@sohu.com
  • 作者简介:陈谱,男,博士,住院医师
  • 基金资助:
    *国家自然科学基金面上项目(No.81373662,No.81874475),北京中医药大学自主课题(No.2019-JYB-XS-232)

Effects of Tongluo Zhitong Ningjiao on synovial membrane inflammation severity of KOA rabbit model at different stages*

Chen Pu, Ruan Anmin, Zhou Jun, Zhang Xiaozhe, Peng Haoxuan, Yang Tongjie, Yang Yi, Cai Gaokai, Wang Qingfu#   

  1. Beijing University of Chinese Medicine Third Affliated Hospital, Beijing 100029, China
  • Received:2020-06-11 Online:2020-12-30 Published:2021-01-05
  • Contact: Prof. Wang Qingfu,Chief Physician,Doctoral Supervisor. Beijing University of Chinese Medicine Third Affliated Hospital. No.51 Anwai Xiaoguan Street, Chaoyang District, Beijing 100029. E-mail: qingpu-wang@sohu.com
  • Supported by:
    National Natural Science Foundation of China (No. 81373662, No. 81874475)

摘要: 目的 观察通络止痛凝胶对不同程度膝关节骨性关节炎(KOA)家兔滑膜炎性程度的影响,探讨通络止痛凝胶治疗KOA的作用机制。方法 将56只家兔随机等分为8组:正常组、假手术组、轻度KOA组、中度KOA组、重度KOA组、轻度KOA通络止痛凝胶干预组、中度KOA通络止痛凝胶干预组、重度KOA通络止痛凝胶干预组。除正常组及假手术组外,其余各组均予以Hulth法建立KOA模型,轻、中、重度KOA组分别于造模后4、8、16周处死,轻、中、重度KOA通络止痛凝胶干预组分别在治疗3周后处死。HE染色观察各组滑膜炎性程度变化;ELISA法检测血清中IL-1β、IL-6、肿瘤坏死因子α(TNF-α)及前列腺素2(PGE2)含量;qPCR和Western blot法检测滑膜组织炎性因子IL-1β、TNF-α及基质金属蛋白酶13(MMP-13) mRNA及蛋白表达。结果 轻、中、重度KOA组较正常组及假手术组滑膜层增厚,炎性浸润增加,予以通络止痛凝胶干预后轻、中、重度KOA家兔滑膜炎性程度均有明显改善。通络止痛凝胶可降低轻、中、重度KOA家兔血清中IL-1β、IL-6、TNF-α及PGE2含量(P<0.05或P<0.01),抑制IL-1β、TNF-α及MMP-13表达(P<0.05或P<0.01)。结论 通络止痛凝胶可以抑制不同程度KOA模型家兔的滑膜炎症,降低炎性因子表达。

关键词: 通络止痛凝胶, 骨关节炎, 滑膜炎症, 炎性因子,

Abstract: Objective To observe the effects of Tongluo Zhitong Ningjiao (Collateral-unblocking and Pain-relieving Gel, TLZTNJ) on the synovial membrane inflammation severity in rabbit models of knee osteoarthritis (KOA) at different stages, and explore its mechanism in the treatment of KOA. Methods 56 rabbits were randomly divided into 8 groups: normal group, sham operation group, mild KOA group, moderate KOA group, severe KOA group, mild KOA group treated with TLZTNJ, moderate KOA group treated with TLZTNJ, and severe KOA group treated with TLZTNJ. Except for the normal group and the sham operation group, KOA models in the other groups were established with the Hulth method. The mild KOA group, the moderate KOA group, and the severe KOA group were executed in the 4th, , 8th, and 16th, week respectively after the models were established. The mild, moderate, and severe KOA groups treated with TLZTNJ were treated with the gel for 3 weeks after the mild, moderate, and severe KOA groups were executed and were executed too. The degree of synovial inflammation was observed by HE staining. The levels of IL-1β, IL-6, TNF-α and PGE2 in serum were detected by ELISA. The mRNA and protein expressions of IL-1β, TNF-α and MMP-13 in synovial tissue were detected by qPCR and WB. Results The synovial layer of rabbits in the mild, moderate, and severe KOA groups significantly thickened, and the inflammatory infiltration increased. After the intervention with TLZTNJ, the severity of synovial inflammation in the mild, moderate, and severe KOA rabbit groups treated with TLZTNJ were significantly reduced. TLZTNJ lowered the levels of IL-1β, IL-6, TNF-α and PGE2 in the serum (P<0.05, P<0.01) and inhibited the expression levels of IL-1β, TNF-α and MMP-13 (P<0.05, P<0.01) of the rabbits with mild, moderate, and severe KOA. Conclusion TLZTNJ can significantly inhibit synovial membrane inflammation and reduce the expression of inflammatory factors in rabbit KOA models at different stages.

Key words: Tongluo Zhitong Ningjiao, osteoarthritis, synovial membrane inflammation, inflammatory factor, rabbits

中图分类号: 

  • R285.5