主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 18-21.doi: 10.3969/j.issn.1006-2157.2014.01.005

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Influences of Qizhi Yifei Capsule medicated serum on expressions of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA in lung fibroblasts stimulated by TGF-β1

FENG Cui-ling1, LIU Zhi-kun1, HOU Ya-jing2, GAO Yong-hong1, SUN Yi-kun1   

  1. 1 Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700;
    2 Shanxi Hospital of Integrated Traditional and Western Medicine
  • Received:2013-10-21 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To definite the regulation effect of Qizhi Yifei Capsule medicated serum on signal transduction pathway of TGF-β1/Smads of fibroblasts in normal rats. Methods The fibroblasts were separated from lung tissue in rats after birth for 2-3 days, and then randomly divided into blank group, model group and medicated serum group. Firstly model group and medicated serum group were given DMEM (containing 2.5 ug/L TGF-β1) and blank group was given fresh non-serum DMEM, and secondly blank group and model group were given DMEM with blank serum (5%) and medicated serum group was given DMEM with Qizhi Yifei Capsule medicated serum (5%). The expressions of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR) after 48 h and 72 h. Results In model group, the expressions of Smad3 mRNA and Smad4 mRNA increased after 48 h, and those of Smad3 mRNA, Smad4 mRNA and Smad7 mRNA increased after 72 h compared with blank group (P<0.05). In medicated serum group, the expressions of Smad3 mRNA and Smad4 mRNA decreased and expression of Smad7 mRNA increased after 48 h, and the expressions of Smad3 mRNA and Smad4 mRNA decreased after 72 h compared with model group (P<0.05). Conclusion Qizhi Yifei Capsule can inhibit the proliferation of lung fibroblasts stimulated by TGF-β1 through regulating the conduction of signal transduction pathway of TGF-β1/Smads.

Key words: chronic obstructive pulmonary disease, cell culture, transforming growth factor-β1/Smads signal pathway, fibroblasts, Qizhi Yifei Capsule medicated serum

CLC Number: 

  • R285.5