主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 22-26.doi: 10.3969/j.issn.1006-2157.2014.01.006

Previous Articles     Next Articles

Protective effect of main component combinations of Fuzi (Radix Aconiti Lateralis praeparata) and Gancao (Radix Glycyrrhizae) on passage myocardial cells injured by aconitine in rats

ZHOU Tian-mei1, YANG Jie-hong2, WAN Hai-tong2, ZHANG Yu-yan2, ZHOU Hui-fen2   

  1. 1 Guangxing Hospital Affiliated to Zhejiang Chinese Medical University, Zhejiang 310007;
    2 Zhejiang Chinese Medical University
  • Received:2013-06-26 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To investigate the protective effects and mechanism of the main component combinations of Fuzi (Radix Aconiti Lateralis praeparata) and Gancao (Radix Glycyrrhizae) on passage myocardial cells injured by aconitine in rats. Methods The influences of main component combinations of Fuzi and Gancao on survival rate of myocardial cells were detected by using methyl thiazolyl tetrazolium test (MTT), and activities of succinodehydrogenase (SDH), Na+-K+ ATPase and Ca2+-Mg2+ ATPase were determined by using kits of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase. Results In aconitine group (120 umol/L), survival rate of myocardial cells and activities of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase decreased significantly. In all combination groups, survival rate of myocardial cells and activities of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase were recovered in varying degrees (P<0.05 or P<0.01). The recovery of survival rate of myocardial cells was the most significant in combination group 3 (P<0.01), recovery of SDH activity was the most significant in combination group 5 (P<0.01), and recoveries of Na+-K+ ATPase and Ca2+-Mg2+ ATPase were the most significant in combination group 4 (P<0.01) compared with verapamil group (P<0.01 or P<0.05). Conclusion The main component combinations of Fuzi and Gancao can protect myocardial cells from injury and play decreasing toxicity role through regulating the energy metabolism of SDH, Na+-K+ ATPase and Ca2+-Mg2+ ATPase.

Key words: Fuzi (Radix Aconiti Lateralis praeparata), Gancao (Radix Glycyrrhizae), combination, aconitine, passage myocardial cells, succinodehydrogenase, rats

CLC Number: 

  • R285.5