主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 27-32.doi: 10.3969/j.issn.1006-2157.2014.01.007

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Evolvement rule and biological basis of TCM syndromes in rat depression model induced by chronic stress or reserpine antagonisim

LI Yu-bo, SUN Yu-xiu, MA Xue-ling, XUE Xiao-xing, CHAI Xin-lou, CHEN Jian-xin, ZHAO Hui-hui, GUO Shu-zhen, WANG Wei-ming, WANG Wei   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2013-07-15 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To compare rat depression model induced by chronic unpredictable mild stress (CUMS) or reserpine antagonisim, and describe the evolvement rule and initially explain molecular mechanism from the angle of TCM syndromes. Methods The rat depression model was established by applying CUMS or intraperitoneal injection of reserpine. The syndromes were distinguished in rats through ethological determination and equivalent transforming from clinical symptoms to macroscopic surface features. The content of serum noradrenalin (NE) was detected by using enzyme-linked immunosorbent assay (ELISA) and content of 5-hydroxytryptamine (5-HT) in cerebral hippocampal CA2 zone was detected by using immunohistochemistry technique. Results The evolvement rule of syndromes showed as follows: CUMS group had syndrome of liver-qi depression 2 weeks after stress and syndrome of liver depression and spleen deficiency 6 weeks after stress, and reserpine group had syndrome of spleen-qi deficiency 2 weeks after injecting and syndrome of liver depression and spleen deficiency 4 weeks after injecting. The level of serum NE increased in CUMS group (P<0.05) after 4 weeks and decreased (P<0.05) after 8 weeks, and continuously decreased (P<0.05) in reserpine group. The level of brain 5-HT was significantly lower in CUMS group and reserpine group than that in normal group (P<0.05), but there was no significant difference between CUMS group and reserpine group (P>0.05). Conclusion The syndromes of depression model induced by CUMS develop gradually from syndrome of liver-qi depression to syndrome of liver depression and spleen deficiency, and those of depression model induced by reserpine antagonisim, from syndrome of spleen-qi deficiency to syndrome of liver depression and spleen deficiency. The difference in the evolvement of syndromes is correlated to the levels of 5-HT and NE.

Key words: depression, chronic unpredictable mild stress, reserpine, TCM syndromes, 5-hydroxytryptamine, noradrenalin, rats

CLC Number: 

  • R285.5