主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 43-47.doi: 10.3969/j.issn.1006-2157.2014.01.010

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Influence of emodin on contraction of in vitro ileum smooth muscle in rats

GAO Liu1, HU Pan1, HAN Xiao1, JIANG Li-li1, YANG Jing1, MA Hui-jie1, QIAO Xiao-wen2, CHANG Ai-rong3, ZHOU Ming-zhong4   

  1. 1 Section of Physiology, Hebei Medical University, Hebei 050017;
    2 Department of Pediatrics, Chinese Medical Hospital of Xinji City;
    3 Department of Neurology, Workers’Hospital, CSCEC, Tangshan City;
    4 Health Inspection, Health Bureau of Kaiping District, Tangshan City
  • Received:2013-02-19 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To observe the influence of emodin on contraction of in vitro ileum smooth muscle in rats, and investigate the mechanism. Methods The samples of in vitro ileum were randomly divided into control group, 1 μmol/L emodin group, 5 μmol/L emodin group, 10 μmol/L emodin group, 20 μmol/L emodin group, propranolol+emodin group (PRO+emodin group), glibenclamide+emodin group (GLI+emodin group), L-NAME+emodin group, calcium-free control group and calcium-free emodin group (each n=6). The rats were sacrificed by applying cervical dislocation for isolating the ileum. The samples of ileum segments were connected with tension transducer, which were then put into oxygen saturation K-H solution. The influences of emodin on contraction tension (TE), amplitude (AM) and frequency (FR) of ileum smooth muscle were recorded by using BL-420+ biological signal processing system. Results Emodin reduced significantly contraction AM of rat ileum smooth muscle (P<0.05 or P<0.01) showing a concentration dependence, and had no significant influences on contraction FR and TE. Propranolol (P<0.01) and glibenclamide (P<0.01) partially blocked up the inhibitory effect of emodin on ileum smooth muscle, but L-NAME had no effect. The contraction of ileum smooth muscle induced by calcium chloride could be inhibited by emodin (P<0.05). Conclusion Emodin can significantly attenuate the contraction AM of rat ileum smooth muscle and has no effects on contraction TE and FR, which may be related to activating β-adrenergic receptor, exciting ATP sensitive potassium channel and inhibiting extracellular calcium influx.

Key words: emodin, ileum smooth muscle, β-adrenergic receptor, ATP sensitive potassium channel, extracellular calcium influx, rats

CLC Number: 

  • R285.5