主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 48-52.doi: 10.3969/j.issn.1006-2157.2014.01.011

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Influences of eye acupuncture on expressions of BDNF and p75NTR and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury

MA Xian-de1, WANG Shou-yan1, ZHANG Wei2   

  1. 1 Teaching and Experiment Center, Liaoning University of Traditional Chinese Medicine, Liaoning 110032;
    2 First Affiliated Hospital of Liaoning University of Traditional Chinese Medicine
  • Received:2013-07-19 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To observe the influences of eye acupuncture on expressions of brain-derived neurotrophic factor (BDNF) and p75 neurotrophic receptor (p75NTR) and its cerebral protective effect in rats with cerebral ischemia-reperfusion injury, and discuss the therapeutic mechanism of eye acupuncture. Methods SD rats (n=62) were randomly divided into blank group (n=11), sham-operation group (n=11)and model copy group (n=40). The model of cerebral ischemia-reperfusion was established by using suture method, and successfully modeled rats (n=32) were divided into model group and eye acupuncture group. The eye acupuncture group was given needling in the acupoints in areas of liver, upper energizer, lower energizer and kidney according to the acupoint selection methods in human body after reperfusion for 2 h, once every 8 h for 10 times. The rats were killed 30 min after the last needling for peeling ischemic half dark cerebral tissue, and the gene and protein expressions of BDNF and p75NTR were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry technique. Results Compared with blank group, the gene and protein expressions of BDNF and p75NTR increased significantly in model group (P<0.01 or P<0.05). Compared with model group, the gene and protein expressions of BDNF increased significantly (P<0.01), and gene and protein expressions of p75NTR decreased significantly (P<0.01 or P<0.05) in eye acupuncture group. Conclusion The expressions of BDNF and p75NTR will increase for antagonizing brain injury after cerebral ischemia-reperfusion. Eye acupuncture can protect brain through up-regulating the expression of BDNF and down-regulating the expression of p75NTR.

Key words: eye acupuncture, cerebral ischemia-reperfusion, nerve growth factor, p75 neurotrophic receptor, rats

CLC Number: 

  • R285.5