主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (1): 53-57.doi: 10.3969/j.issn.1006-2157.2014.01.012

Previous Articles     Next Articles

Influences of needling chorea-tremble controlled zone on expressions of dopaminergic neurons and BDNF in mice with Parkinson’s disease

FENG Jing, SUN Hong-mei, WANG Yuan-yuan, XU Hong, WU Hai-xia, HE Xin, GAO Yu-shan, ZHANG Shu-jing, GONG Xiao-gang   

  1. School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029
  • Received:2013-05-28 Online:2014-01-30 Published:2014-01-30

Abstract: Objective To investigate the protective effect and possible mechanism of acupuncture on dopaminergic neurons (DA neurons) through observing the influences of needling chorea-tremble controlled zone on expression of substantia nigra brain derived neurotrophic factor (BDNF) in mice with Parkinson’s disease (PD). Methods Male C57BL/6 mice were randomly divided into normal group, model group, needling group, madopar group and needling+madopar group. The later 4 groups were given intraperitoneal injection of MPTP for establishing PD model, and then the later 3 groups were given corresponding treatment for 28 d. The ethological changes were observed in mice by using climbing method. The pathological changes of midbrain substantia nigra dopaminergic neurons were observed after HE staining. The loss of DA neurons was detected by applying immunohistochemistry technique and expression of BDNF was detected by using Western blot method. Results The ethological scores increased significantly in needling group, madopar group and needling+madopar group compared with model group (P<0.05). The count of DA neurons was higher in model group than that in normal group (P<0.05), and was higher in needling+madopar group than that in madopar group (P<0.05). The expression of BDNF decreased significantly in model group compared with normal group (P<0.05), and increased significantly in needling+madopar group compared with madopar group (P<0.05). Conclusion Needling chorea-tremble controlled zone combining madopar can treat PD through increasing the expression of BDNF and decreasing the loss of DA neurons in mice with PD.

Key words: acupuncture, mice, Parkinson’s disease, chorea-tremble controlled zone, brain derived neurotrophic factor

CLC Number: 

  • R285.5