主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (2): 90-93.doi: 10.3969/j.issn.1006-2157.2014.02.005

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Active fraction of Huanglian Jiedu Tang affecting expression of penumbra neurons NeuN,Caspase-3 and PARP in rats after cerebral ischemia

LONG Jian-fei1, ZHANG Chi2, ZHANG Qiu-xia1, WANG Lei1, ZHAO Hui1, BIAN Bao-lin3, ZHAO Hai-yu3   

  1. 1 School of Traditional Chinese Medicine, Capital University of Medical Sciences, Beijing 100069;
    2 Beijing University of Chinese Medicine;
    3 Institute of Chinese Meteria Medica, China Academy of Chinese Medical Sciences
  • Received:2013-10-15 Online:2014-02-28 Published:2014-02-28

Abstract: Objective To observe the influences of Huanglian Jiedu tang’s active fractions, which consists of total alkaloids, total flavonoids and total iridoids, on the expressions of penumbra neuronal nuclei (NeuN), cysteine aspartic acid proteinase 3 (Caspase-3) and poly-ADP-ribose polymerase (PARP) in rats after cerebral ischemia, and to discuss the mechanism of protective effects of these fractions on neurons. Methods The rat model of permanent middle cerebral artery occlusion (pMCAO) was chosen. Male SD rats were randomly divided into sham-operation group, model group, aqueous extract group (800 mg/kg), total alkaloids group (44 mg/kg), total flavonoids group (50 mg/kg) and total iridoids group (80 mg/kg). All groups were orally given corresponding medicinals once a day for 7 d. The expressions of NeuN, Caspase-3 and PARP were detected by using immunofluorescence staining combined image analysis technique. Results In model group, the number of NeuN positive cells decreased in ischemic penumbra cortex decreased, expression of NeuN decreased, and expressions of Caspase-3 and PARP increased significantly compared with sham-operation group (P<0.01). In aqueous extract group, total alkaloids group, total flavonoids group and total iridoids group, the number of NeuN positive cells increased in ischemic penumbra cortex, expression of NeuN increased, and expressions of Caspase-3 and PARP decreased compared with model group (P<0.05). Conclusion The aqueous extract, total alkaloids, total flavonoids and total iridoids in Huanglian Jiedu Tang have protective effect on neurons in ischemic penumbra cortex through depressing the activity of Caspase-3 and decreasing the over-expression of PARP.

Key words: Huanglian Jiedu Tang, cerebral ischemia, neuronal nuclei, cysteine aspartic acid proteinase 3, poly-ADP-ribose polymerase, rats

CLC Number: 

  • R285.5