主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (2): 103-106.doi: 10.3969/j.issn.1006-2157.2014.02.008

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Influences of external dryness on neutrophil elastase and IL-10 in airway

NI Sheng1, DING Jian-zhong1, ZHANG Liu-tong2, XIAO Chang-yi3, ZHANG Yan-xiang1   

  1. 1 Medical School, Changjiang University, Hubei 434023;
    2 Hubei College of Chinese Medicine;
    3 Medical School, China Three Gorges University
  • Received:2013-07-27 Online:2014-02-28 Published:2014-02-28

Abstract: Objective To investigate the influence of external dryness on proinflammatory cytokines and anti-inflammatory cytokines in airway and the significance. Methods Kunming mice (n=72) were randomly divided into normal-temperature and normal-dampness group, warm-dryness group and cool-dryness group (each n=24). The mouse model was established by the synthetic stimulation of temperature-relevant humidity-wind. On the 6th day and the 12th day the histopathological changes of respiratory tract and lung ultrastructure were observed, and the content of neutrophil elastase (NE), α1-antitryptase (α1-AT), interlukin-10 (IL-10), tumor necrosis factor-α (TNF-α), IgG and secretory IgA (sIgA) in bronchoalveolar lavage fluid were detected. Results There were squamous metaplasia of airway epithelium and cilium function defect, blood stasis and edema in pulmonary alveoli accompanied by pulmonary emphysema in warm-dryness group and cool-dryness group compared with normal-temperature and normal-dampness group on the 6th and 12th day. The observation on lung ultrastructure indicated that the number of mitochondria and osmiophilic multilamellar body of alveolar type II (ATⅡ) decreased in warm-dryness group and cool-dryness group, meanwhile the level of NE increased, while the levels of α1-AT and IL-10 decreased (P<0.05). In warm-dryness group the levels of IgG and sIgA were decreased (P<0.01). In cool-dryness group the level of IgG decreased but the changes of sIgA level had no significance. Conclusion External dryness can increase NE and cause dysfunction of down-modulatory role of IL-10 in airway and impair immune defending function of airway, which is related to pathogenesis of pulmonary injury.

Key words: external dryness, neutrophil elastase, interlukin-10, mice

CLC Number: 

  • R285.5