主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (2): 112-115.doi: 10.3969/j.issn.1006-2157.2014.02.010

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Influences of Yiqi Huoxue Fang and Bushen Shengsui Fang on genetic and protein expressions of Ctnnb1 and Krt1 in rats with focal cerebral ischemia/reperfusion

XU Wei, HU Jian-peng, WANG Jian, WU Sheng-bing, WANG Li-na, HE Ling, JIAN Wei, TAN Hui   

  1. Key Laboratory of Xin’an Medicine Jointly Developed by Ministry of Education and Anhui Province, Anhui University of Traditional Chinese Medicine, Anhui 230038
  • Received:2013-08-11 Online:2014-02-28 Published:2014-02-28

Abstract: Objective To investigate the influences of Yiqi Huoxue Fang and Bushen Shengsui Fang on the genetic and protein expressions of Ctnnb1 and Krt1 in rats with focal cerebral ischemia/reperfusion. Methods The rat model of focal cerebral ischemia/reperfusion was established. The rats were randomly divided into sham group, model group, Yiqi Huoxue Fang group (Y group) and Bushen Shengsui Fang group (B group). After cerebral ischemia for 2 h and reperfusion, the genetic expressions of Ctnnb1 and Krt1 in ischemic frontoparietal cortex were detected by using PCR assay, and protein expressions of Ctnnb1 and Krt1 in ischemic frontoparietal cortex and hippocampus CA1 zone were detected by using immunohistochemistry Envision two-step method. Results The genetic expressions of Ctnnb1 and Krt1 increased significantly in model group compared with sham group (P<0.05), and decreased significantly in Y group compared with model group (P<0.05). The genetic expression of Ctnnb1 decreased significantly in B group (P<0.05), which had significant difference compared with Y group (P<0.05). The protein expressions of Ctnnb1 and Krt1 in frontoparietal cortex and hippocampus CA1 zone increased significantly in model group compared with sham group (P<0.05), and decreased significantly in Y group compared with model group (P<0.05). The protein expression of Ctnnb1 was significantly lower in Y group than that in B group (P<0.05). Conclusion Yiqi Huoxue Fang and Bushen Shengsui Fang can improve the repairing of injured cerebral tissue through reducing the the genetic and protein expressions of Ctnnb1 and Krt1 in cortex and hippocampus and regulating notch signal pathway.

Key words: yiqi Huo xue Fang, Bushen Shengsui Fang, cerebral ischemia/reperfusion, hippocampus CA1 zone Ctnnb1, Krt1, rats

CLC Number: 

  • R285.5