主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (2): 116-120.doi: 10.3969/j.issn.1006-2157.2014.02.011

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Protective effect of hedysarum polybotrys polysacchcaide on kidney in db/db mice with diabetic nephropathy and its influence on expressions of renal PKCα and VEGF

WEI Yu-jiao1, JIN Zhi-sheng1, ZHU Zhen-ling1, ZHENG Li-hong2, LIN Hai-long1, GUAN Yan1, QI Xue-yan1, CHU Hui-yuan1   

  1. 1 Gansu College of Traditional Chinese Medicine, Gansu 730000;
    2 Second Affiliated Hospital of Heilongjiang University of Chinese Medicine
  • Received:2013-08-09 Online:2014-02-28 Published:2014-02-28

Abstract: Objective To discuss the protective mechanism of hedysarum polybotrys polysacchcaide (HPS) on kidney in mice with diabetic nephropathy (DN) through studying the influences of HPS on renal protein kinase Cα (PKCα) and vascular endothelial growth factor (VEGF) in db/db mice with DN. Methods Male db/db mice (n=50, 12 weeks old) were randomly divided into five groups: low-dose, mid-dose and high-dose HPS groups (low-dose group, mid-dose group and high-dose group), enalapril group, model group. The mice in normal group (n=10) were db/m mice of the same age. The mice in all groups were given intervention respectively for 8 w. The concentration of blood glucose was detected before intervention and at the end of the 2nd w, 4th w, 6th w and 8th w after intervention. The mice were killed at the end of 8th w after 24-h urine was collected and 24-h urine protein excretion quantity was detected. The levels of serum creatinine (Scr) and blood urea nitrogen (BUN) were detected. The mRNA and protein expressions of renal PKCα and VEGF were detected by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Results The level of blood glucose decreased in HPS groups after 8 w compared with model group (P>0.05), and Scr, BUN and 24-h urine protein excretion quantity decreased significantly compared with model group (P<0.05). There was no significant difference in protein expression between low-dose group and model group (P>0.05). The protein expression in all intervention groups decreased compared with model group (P<0.05), which was more significant in high-dose group (P<0.01). Conclusion HPS has a therapeutic effect on DN in db/db mice with type 2 diabetes, while the effect dose not dependent on hyperglycemic. HPS may slow down DN progression by inhibiting the excessive expression of PKCα and VEGF.

Key words: diabetic nephropathy, hedysarum polybotrys polysacchcaide, protein kinase Cα, vascular endothelial growth fattor db/db mice

CLC Number: 

  • R285.5