主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (4): 231-235.doi: 10.3969/j.issn.1006-2157.2014.04.004

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Improving proliferation of ovarian granulocytes by therapy of tonifying kidney and activating blood in rats with follicular development disorder

CAI Jing1, WU Ke-ming2, XIONG Ting-ting2, HUANG Li2, LIAO Feng-jiao2   

  1. 1 Affiliated Hospital of Shaanxi University of Chinese Medicine, Shaanxi 712000;
    2 Chengdu University of Traditional Chinese Medicine
  • Received:2013-09-15 Online:2014-04-30 Published:2014-04-30

Abstract: Objective To investigate the melacular mechanism of Xinjia Guishen Wan (a compund formular with the actions of tonifying kidney and activating blood) in promoting follicular development taken the proliferation of granulocytes as an entry point. Methods The model of follicular development disorder was established by using GTW in rats, and then the rats were treated with high-dose, mid-dose and low-dose Xinjia Guishen Wan for 2 weeks. The ovary index was calculated, and granulocyte thickness was detected with light microscope after HE staining. The expressions of ovary CyclinD2 mRNA and granulocyte CyclinD2 protein were detected respectively by using Taqman RT-PCR and immunohistochemistry method. Results The ovary index, granulocyte thickness and expressions of ovary CyclinD2 mRNA and granulocyte CyclinD2 protein all decreased in model group compared with normal group (P<0.05 or P<0.01). After treatment with Xinjia Guishen Wan in different doses, the above-mentioned indexes all increased especially in high-dose group and mid-dose group (P<0.05 or P<0.01). Conclusion Xinjia Guishen Wan can accelerate the progress of cell cycle, promote granulocyte proliferation, and further improve follicular development through up-regulate the expression of CyclinD2.

Key words: tonifying kidney and activating blood, Xinjia Guishen Wan, granulocyte thichness, CyclinD2, follicular development disorder, rats

CLC Number: 

  • R285.5