主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (4): 236-239.doi: 10.3969/j.issn.1006-2157.2014.04.005

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Influences of curcumin on carbohydrate metabolism and differentiation of insulin-resistant 3T3-L1 preadipocytes

QIN Pei-jie, ZHANG Dong-wei, MO Fang-fang, ZHAO Dan-dan, LI Xiao-ke, FANG Xin, MU qian-qian, YU Na, GAO Si-hua   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2013-09-15 Online:2014-04-30 Published:2014-04-30

Abstract: Objective To discuss the influences of curcumin on carbohydrate metabolism and differentiation of preadipocytes and mechnism. Methods The model of adipocyte insulin-resistant was established by using culturing-inducing combination method of high-dose glucose and insulin. The uptake of 3H-glucose was detected in adipocytes after inventioned with curcumin, and the differentiation degree of adipocytes was analyzed by using spectrophotography quantitatively after oil red O staining. The expressions of PPARγ and C/EBPα were detected by using real-time fluorescence quantitative polymerase chain reaction (RT-PCR). Results The uptake rate of glucose was 134.51% in curcumin group compared with control group, and the differentiation of adipocytes and expressions of PPARγ mRNA and C/EBPα mRNA were improved significantly compared with control group (P<0.05). Conclusion Curcumin can improve glucose uptake and differentiation of adipocytes and expressions of PPARγ mRNA and C/EBPα mRNA.

Key words: adipocytes, curcumin, carbohydrate metabolism, differentiation

CLC Number: 

  • R285.5