主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (4): 259-262.doi: 10.3969/j.issn.1006-2157.2014.04.011

Previous Articles     Next Articles

Quantity determination of 7 kinds of organic acid in Acanthopanax sessiliflorus and comparison among Acanthopanax sessiliflorus, Acanthopanax gracilistylus and Acanthopanax senticosus

SONG Yang, FENG Xue-song   

  1. Department of Pharmaceutical Analysis, School of Pharmacy China Medical University, Liaoning 110001
  • Received:2013-09-27 Online:2014-04-30 Published:2014-04-30

Abstract: Objective To establish a HPLC method for simultaneous determination of 7 kinds of organic acid in Acanthopanax sessiliflorus (chlorogenic acid, neochlorogenic acid, caffeic acid, 1,3-dicaffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid). Methods The HPLC procedure was performed on the chromatographic column of Hypersil ODS2 C18 (250×4.6 mm, 5 μm) at room temperature, and mobile phase was 0.1% formic acid in acetonitrile-0.1% formic acid in water. The flow velocity was 1.0 mL/min and detection wavelength was 324 nm. Results All 7 kinds of organic acid showed good linear relationship respectively at range of 20.5-328 μg/mL, 1.99-31.8 μg/mL, 2.43-38.9 μg/mL, 2.44-39.0 μg/mL, 2.52-40.3 μg/mL, 7.25-116 mg/L and 2.42-38.7 μg/mL. The average recoveries of them were within a range from 95.2% to 103.9%. Conclusion The method is quick, simple, accurate and sensitive, which can be used as a method for controlling the quality of Acanthopanax sessiliflorus and other Acanthopanax materials.

Key words: Acanthopanax sessiliflorus, Acanthopanax gracilistylus, Acanthopanax senticosus, organic acid, quantity determination, HPLC

CLC Number: 

  • R284.1