主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (4): 263-268.doi: 10.3969/j.issn.1006-2157.2014.04.012

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Distribution of norcantharidin determined by LC-MS/MS in rat tissues

LI Wu-chao1, LIU Min-chen2, MA Xiao-qiong3, WEI Ying-hui1, ZHANG Rong-rong1, SUN Yue1, LI Fan-zhu1   

  1. 1 College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Zhejiang 310053;
    2 College of Pharmaceutical Sciences, Zhejiang University;
    3 National Clinical Research Base of Traditional Chinese Medicine, First Affiliated Hospital, Zhejiang Chinese Medical University
  • Received:2013-10-25 Online:2014-04-30 Published:2014-04-30

Abstract: Objective To establish a analysis method of LC-MS/MS to study the internal change features of norcantharidin (NCTD) in high dose, medium dose and low dose. Methods The HPLC procedure was performed on the chromatographic column of CAPCELL PAK MG II C18 at room temperature. The mobile phase was acetonitrile-water (containing 0.1% formic acid) in gradient elution, and flow velocity was 0.2 mL/min. A tandem mass spectrometer equipped with electrospray ionization (ESI) source was used for detecting in the mode of multiple reaction monitoring (MRM, ion pair m/z 169.3→123.1 for NCTD and ion pair m/z 267.1→135.1 for internal standard ribavirin). The concentration of NCTD was detected in different rat tissues by using LC-MS/MS. Results The linear range of NCTD was (0.01-10.00) μg/mL in heart, liver and lung, and (0.10-10.00) μg/mL in spleen and kidney. Intra-day RSD and inter-day RSD were less than 14% in all groups and extraction recovery was between 81.0% and 106%. After intravenous injection of NCTD in 3 dose levels, the dynamic changes in all tissues (except of spleen) were similar. The concentration of NCTD reached to the peak after one hour and then rapidly decreased. The distribution level of NCTD was higher in liver and kidney showing a dose-dependent manner. Conclusion The LC-MS/MS method is simple, sensitive and accurate, which can be used for study the distribution of NCTD in rat tissues. When dose of NCTD is higher, its concentration is also higher in kidney, which may be related to its renal toxicity. The study will provide a reference for rational administration of NCTD in clinic.

Key words: norcantharidin, LC-MS/MS, distribution in tissues, rats

CLC Number: 

  • R284.1