主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (5): 300-303.doi: 10.3969/j.issn.1006-2157.2014.05.003

Previous Articles     Next Articles

Influence of Qingkailing Injection on biofilm of Staphylococcus aureus

HAN Xue1,2, WANG Yi2, WANG Yu-nong1, LI Dong3, LIU Chong4, ZHANG Te-li3, WANG Yi-zhu5, LIU Jun-feng3, HAO Yu1   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029;
    2 Experimental Research Center, China Academy of Chinese Medical Sciences;
    3 Shenwei Pharmaceutical Group LTD.;
    4 College of Life Sciences, Beijing Normal University;
    5 Basic Medical Sciences College, Jilin University
  • Received:2014-01-02 Online:2014-05-30 Published:2014-05-30

Abstract: Objective To observe the influence of Qingkailing Injection on biofilm of Staphylococcus aureus (S. aureus), and study its antibacterial mechanism. Methods The biofilm of S. aureus was cultured on 96-well plate at formation stage and mature stage. The amount of living S. aureus in biofilm was determined by using Microbial Viability Assay Kit-WST dyeing after OD value obtained from 450 nm by using Microplate Reader. The destructive effect of Qingkailing Injection on measure biofilm was observed by using BioFlux biofilm system, and the changes of mature biofilm structure and S. aureus morphology after medication were observed by using scanning electron microscope. Results Qingkailing Injection reduced the amount of living S. aureus in biofilm at formation stage (22 500 mg/L-352 mg/L), and reduced the amount of the amount of living S. aureus in biofilm at mature stage (22 500 mg/L-1 406 mg/L, P<0.05). The results of BioFlux biofilm system showed that Qingkailing Injection destructed the structure of measure biofilm of S. aureus, and the results of scanning electron microscope showed that Qingkailing Injection induced biofilm loose and S. aureus in different sizes. Conclusion Qingkailing Injection can inhibit the biofilm formation and destruct mature biofilm of S. aureus, so it has the anti-effect on the biofilm of S. aureus.

Key words: Qingkailing Injection, Staphylococcus aureus, biofilm

CLC Number: 

  • R285.5