主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (5): 304-308.doi: 10.3969/j.issn.1006-2157.2014.05.004

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Effective mechanism of iridoid constituents of Zhizhuxiang (Jatamans Valeriana Rhizome) in rats with irritable bowel syndrome

SHI Rui-rui, WANG Juan, YAN Xing-li, HU Jing-hong, GAO Zeng-ping, JIA Ming-xian, YU Xue, WANG Jing   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2014-01-05 Online:2014-05-30 Published:2014-05-30

Abstract: Objective To investigate the influences of iridoid of Zhizhuxiang (Jatamans Valeriana Rhizome) on the changes of 5-hydroxytryptamine (5-HT), tryptophan hydroxylase 1 (TPH1) and monoamine oxidase-A (MAO-A) in rats with irritable bowel syndrome (IBS). Methods Male SD rats were randomly divided into control group, model group, pinaverium group, and high-dose (0.6 mg/kg), mid-dose (0.3 mg/kg) and low-dose (0.15 mg/kg) iridoid groups (high-dose group, mid-dose group and low-dose group). The rat model of IBS was established by using chronic stress method. The influence of iridoid on the threshold of abdominal withdraw reflection (AWR) was observed in all groups. The content of serum 5-HT was detected by using HPLC-EC, the expression of colon TPH1 mRNA was detected by using RT-PCR, and activity of colon MAO-A was determined by using colorimetric method. Results The pain pressure threshold and maximum tolerance pressure threshold decreased significantly in model group (P<0.01), and increased significantly in 3 iridoid groups (P<0.01). In model group, the content of serum 5-HT increased (P<0.05), expression of colon TPH1 mRNA increased (P<0.01) and activity of MAO-A decreased (P<0.05). In mid-dose group and pinaverium group, the content of serum 5-HT and expression of colon TPH1 mRNA decreased significantly and activity of MAO-A increased significantly (P<0.01 or P<0.05). In low-dose group, the expression of colon TPH1 mRNA decreased significantly (P<0.05) and activity of MAO-A increased significantly (P<0.05). Conclusion Iridoid of Zhizhuxiang can affect the content of serum 5-HT and reduce vislral sensitivity in rats with IBS through regulating colon TPH1 and MAO-A.

Key words: iridoid of Zhizhuxiang (Jatamans Valeriana Rhizome), irritable bowel syndrome, 5-HT, TPH1, MAO-A, rats

CLC Number: 

  • R285.5