主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (5): 330-332.doi: 10.3969/j.issn.1006-2157.2014.05.010

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Influences of Bushen Huoluo Fang on sex hormones and ovarian morphology in rats with polycystic ovary syndrome

LIN Hui1, HUANG Qun2, WAN Ping3, YANG Yan1, FU Feng-chun1, PENG Gui-yang1   

  1. 1 Hunan College of Traditional Chinese Medicine, Hunan 412012;
    2 First Affiliated Hospital of Hunan College of Traditional Chinese Medicine;
    3 College of Integrated Medicine of Chinese and Western Medicine, Hunan University of Chinese Medicine
  • Received:2014-01-10 Online:2014-05-30 Published:2014-05-30

Abstract: Objective To investigate the pharmacologic mechanism of Bushen Huoluo Fang in the treatment of polycystic ovary syndrome (PCOS). Methods Female SD rats (n=60) were randomly divided into blank group, model group, positive drug group, and high-dose, mid-dose and low-dose Bushen Huoluo Fang groups (high-dose, mid-dose and low-dose groups, each n=10). The model of PCOS was established by using insulin combining human chorionic gonadotropin (HCG). Bushen Huoluo Fang (in three doses) and Diane-35 were administrated in corresponding groups respectively for 12 d, and then the levels of serum luteinizing hormone (LH), testosterone (T), follicle-stimulating hormone (FSH) and estradiol (E2) were detected by using radioimmunoassay, and the changes of ovarian morphology were observed with light microscope. Results Compared with model group, the weight of ovary (P<0.05), follicle amount (P<0.05) and levels of serum T and LH (P<0.05) decreased significantly, and histomorphology changes were improved in high-dose and mid-dose groups. Conclusion Bushen Huoluo Fang can promote follicular development and ovulation through reducing the levels of T and LH.

Key words: Bushen Huoluo Fang, sex hormones, ovarian morphology, rats

CLC Number: 

  • R285.5