主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (5): 333-336.doi: 10.3969/j.issn.1006-2157.2014.05.011

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Influences of Chuankezhi Injection on Th17 and Treg in mice with airway inflammation

HUANG Dong-hui1, WANG Hui-xian1, WU Shao-li2, LI Tan-hui3   

  1. 1 Guangdong Provincal Hospital of Traditional Chinese Medicine, Guangdong 510120;
    2 Panyu District Hospital of Traditional Chinese Medicine, Guangzhou;
    3 Huidong County Hospital of Traditional Chinese Medicine
  • Received:2014-01-13 Online:2014-05-30 Published:2014-05-30

Abstract: Objective To investigate initially the influences of Chuankezhi Injection on Th17 and Treg in mice with asthma airway inflammation. Methods The model of bronchial asthma was established by applying the intraperitoneal injection of OVA combining nasal drop in BALB/c mice, and then randomly divided into control group, asthma group and Chuankezhi group. Chuankezhi group was intraperitonealy injected Chuankezhi Injection (1.04 ml/kg) and control group and asthma group were intraperitonealy injected normal saline water (200 UL). The changes of lung pathology, levels of BALF IL-17, IL-6, TGF-β1 and IL-10, percentage of CD4+IL-17+ and FOXp3+CD4+CD25+ in peripheral blood, and expressions of lung Foxp3 mRNA, RORγt mRNA and IL-17 mRNA were observed in mice of all groups. Results The pathologic sections of lung tissue showed that there were infiltration of lot eosinophils, lymphocytes and other inflammatory cells around trachea in asthma group, which indicated that model establishment was successful. The level of IL-6 was lower and level of TGF-β1 was higher in Chuankezhi group than those in asthma group (P<0.05). The percentage of CD4+IL-17+ was down-regulated and that of FOXp3+CD4+CD25+ was up-regulated in Chuankezhi group, which was opposite in asthma group (P<0.05). The expression of Foxp3 mRNA was higher and expressions of IL-17 mRNA and RORγt mRNA were lower in Chuankezhi group than those in asthma group (P<0.05). Conclusion Chuankezhi Injection may inhibit the airway infective reaction through reducing the generation of regulatory T cells of FOXp3+ and inhibiting IL-17 release.

Key words: bronchial asthma, Chuankezhi Injection, IL-17, regulatory T cells, airway inflammation

CLC Number: 

  • R285.5