主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (6): 387-392.doi: 10.3969/j.issn.1006-2157.2014.06.007

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Mobilizing effect of panax notoginseng saponins on marrow mesenchymal stem cells in model of cerebral ischemia-reperfusion injury

ZHANG Bao-xia1, ZHANG Jin-sheng2, DU Mei-mei2, WANG Ping3, ZHU Hui-fang2, ZHANG Yang-yang2   

  1. 1 First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Henan 450000;
    2 Third Affiliated Hospital of Henan University of Traditional Chinese Medicine;
    3 Henan Provincial Tumor Hospital
  • Received:2013-12-08 Online:2014-06-30 Published:2014-06-30

Abstract: Objective To investigate the mobilizing effect of panax notoginseng saponins (PNS) on marrow mesenchymal stem cells after cerebral infarction. Methods The rat model of MCAO was established by referencing Zea-Longa method. The modeled rats were randomly divided into model group and high-dose, mid-dose and low-dose Xuesaitong Capsules groups (high-dose group, mid-dose group and low-dose group), and then according to time points divided into 1-d, 7-d, 14-d and 28-d subgroups. High-dose group, mid-dose group and low-dose group were treated with Xuesaitong Capsules (60 g/L, 40 g/L and 20 g/L, 10 g/kg once a day), and normal group and model group were orally given normal saline water once a day for 28 d. The positive percentage of CD54, CD106, CD105 and CD117 in peripheral blood and bone marrow were detected by using flow cytometer at different time points, and expression of stem cell factor (SCF) in peripheral blood and bone marrow was detected by using ELISA at different time points. Results SCF level and positive percentage CD117 and CD105 increased in high-dose group, mid-dose group and model group on the first day, reached the peak on the 14th day, and then decreased gradually, but the increased trend is superior in high-dose group and mid-dose group to model group (P<0.05). The expressions of CD54 and CD106 reached the peak in model group, high-dose group, mid-dose group and model group on the first day and then decreased gradually, and decreased in mid-dose group and high-dose group at all time points compared with model group especially in high-dose group (P<0.05). Conclusion PNS can mobilize marrow mesonchymal stem cells from bone marrow to peripheral blood and promote number of homing stem cells through improve SCF level and decreasing expressions of CD54 and CD106.

Key words: panax notoginseng saponins, marrow mesenchymal stem cells, cerebral infarction, mobilization of stem cells, rats

CLC Number: 

  • R285.5