主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2014, Vol. 37 ›› Issue (6): 397-400.doi: 10.3969/j.issn.1006-2157.2014.06.009

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Influences of Bushen Shengxue Jiedu Fang on expressions of erythropoiesis-related factors in mice with aplastic anemia

DONG Bin, LIU Juan, LOU Li-xia, WU Ai-ming, LV Xi-ying, CHAI Li-min   

  1. Key Laboratory of Chinese Internal Medicine of Ministry of Education and Beijing, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700
  • Received:2013-10-15 Online:2014-06-30 Published:2014-06-30

Abstract: Objective To investigate the influence of Bushen Shengxue Jiedu Fang on erythropoiesis in myelosuppression mice induced by physical and chemical compound factors. Methods BALB/C mice(n=50) were randomly divided into blank group, model group, and low-dose, mid-dose and high-dose Chinese medicinal groups (low-dose group, mid-dose group and high-dose group). The model of aplastic anemia (AA) was established with 60Co-γ ray and cyclophosphamide injection. Bushen Shengxue Jiedu Fang was used for intervention in low-dose, mid-dose and high-dose groups (6.14 g/kg, 12.29 g/kg and 24.57 g/kg). The changes of erythrocyte parameters were detected by using blood cell counter. The level changes of erythrogenin (EPO) and interferon γ (IFNγ) were detected by using ELISA, and expressions of EPO receptor and IFNγ receptor were detected by using RT-PCR. Results Bushen Shengxue Jiedu Fang increased erythrocyte parameters, relieved anemia symptoms, increased the level of serum EPO and improved significantly the expressions of EPO receptor gene in bone marrow cells, decreased the content of IFNγ in peripheral blood, and inhibited the expression of IFNγ receptor gene. Conclusion Bushen Shengxue Jiedu Fang may have curative effect on AA through increasing EPO activity, inhibiting immune reaction induced by IFNγ, recovering normal function of hematopoietic stem cell, and promoting normal differentiation and mature of red blood cells.

Key words: Bushen Shengxue Jiedu Fang, aplastic anemia, erythrocyte parameters, erythrogenin, interferonγ, interferonγ receptor, mice

CLC Number: 

  • R285.5